副猪嗜血杆菌ompP2基因的克隆鉴定及序列分析  

IDENTIFICATION AND SEQUENCE ANALYSIS ON THE OMPP2GENE OF HAEMOPHILUS PARASUIS

作　　者：贾爱卿 王贵平 樊惠英 廖明 JIA Ai-qing;WANG Gui-ping;FAN Hui-ying;LIAO Ming(Key Laboratory for Animal Vaccine Development, Ministry of Agriculture, College of Veterinary Medicine, South China Agricultural University,Guangzhou510642,China;Guangdong Haid Institute of Animal Husbandry & Veterinary, Guangzhou511400,China)

机构地区：[1]华南农业大学兽医学院农业部兽用疫苗创制重点实验室,广州510642 [2]广东海大畜牧兽医研究院,广州511400

出　　处：《中国动物传染病学报》2017年第1期45-49,共5页Chinese Journal of Animal Infectious Diseases

基　　金：农业部公益性行业科研专项经费项目(201303034)

摘　　要：本文用PCR技术对华南地区分离的15株不同血清型副猪嗜血杆菌菌株和2株国际参考菌株的外膜蛋白编码基因ompP2基因进行克隆鉴定，并以CLUSTALS1和PHYLIP-3.68软件将ompP2基因序列进行比对和遗传进化分析。17株副猪嗜血杆菌菌株均能扩出ompP2基因，克隆测序结果发现ompP2基因长度为1077~1092bp，其中菌株H37、H38和H49中P2存在两段34~39个氨基酸的保守插入序列。β折叠预测结果显示H37、H38和H49比其他菌株多一个环结构。遗传进化分析显示H37、H38和H493株含有插入片段的菌株聚为一群，与其他菌株遗传距离相对较远。上述结果为进一步验证ompP2蛋白的功能及相关研究奠定了基础。We used PCR to determine the ompP2gene of15H.parasuis local isolates and2reference strains.Then we sequenced the ompP2gene of H.parasuis.All ompP2gene sequence from H.parasuis,were analyzed for phylogenetic relationship.An approximately1.1kb fragment was obtained from the genomic DNA of17H.parasuis strains.Sequence analysis showed that the size of the ompP2gene was difference,and had two conserved sequences of34～39amino acids in strains H37,H38and H49.Beta folding prediction showed which had more than one ring structure and relatively far distance in strains H37,H38and H49than other strains.These results laid the foundation for further verifi cation of the function and related research of ompP2protein.

关 键 词：副猪嗜血杆菌 ompP2基因 序列分析 进化树 

分 类 号：S852.612[农业科学—基础兽医学]