日本血吸虫高迁移率族蛋白活化鼠巨噬细胞的初步研究  

PRELIMINARY STUDY ON THE ACTIVATION OF MACROPHAGOCYTES BY HIGH MOBILITY GROUP BOX1 PROTEIN OF SCHISTOSOMA JAPONICUM

作　　者：李丹丹[1,2] 马丽贞[1] 苑纯秀[1] 史晓娜[1,2] 艾敏[1] 塔娜[1] 冯新港[1] LI Dan-dan;MA Li-zhen;YUAN Chun-Xiu;SHI Xiao-na;AI Min;TA Na;FENG Xin-gang(Key Laboratory of Animal Parasitology of the Ministry of Agriculture, Shanghai Veterinary Research Institute, CAAS, Shanghai 200241,China;College of Life and Environment Sciences, Shanghai Normal University, Shanghai 200234, China)

机构地区：[1]中国农业科学院上海兽医研究所农业部动物寄生虫学重开放点实验室,上海200241 [2]上海师范大学生命与环境科学学院,上海200234

出　　处：《中国动物传染病学报》2017年第1期59-65,共7页Chinese Journal of Animal Infectious Diseases

基　　金：中国农业科学院动物血吸虫病创新团队基金

摘　　要：为研究日本血吸虫高迁移率族蛋白(Schistosoma japonicum high mobility group box1,Sj HMGB1)体外活化小鼠巨噬细胞的功能,利用真核重组Sj HMGB1蛋白与巨噬细胞共培养48 h,流式细胞术检测巨噬细胞表面标志分子以及趋化因子受体的表达。收集Sj HMGB1蛋白与巨噬细胞共孵育48 h后上清,ELISA检测上清中TNF-α与IL-10的含量,Griess法检测上清中NO的含量。流式结果显示,与对照组相比,Sj HMGB1蛋白刺激组的巨噬细胞表面MHC-Ⅱ分子,TLR4受体以及趋化因子受体CCR7的表达显著上调且差异具有极显著统计学意义(P<0.01),TLR2受体的表达显著下调,差异具有显著统计学意义(P<0.05)。ELISA结果显示,Sj HMGB1能够刺激巨噬细胞分泌致炎因子TNF-α,未能促进抑炎因子IL-10的释放。Griess法表明Sj HMGB1能够促进巨噬细胞分泌NO。本研究结果表明Sj HMGB1可能通过TLR4通路活化小鼠巨噬细胞并诱导其向致炎性M1型巨噬细胞极化。This study aimed at determining the activation of high mobility group box1protein(SjHMGB1)of Schistosoma japonicum on mouse macrophagocyte.When Recombinant SjHMGB1protein from eukaryotic cell expression system was incubated with macrophage for48h,the expression of macrophage surface molecules and chemokine receptors were detected by fl ow cytometry.TNF-αand IL-10in the culture supernatant were detected by ELISA,and the content of NO in supernatant was assayed by Griess method.Results showed that,compared with the control group,the expression levels of MHC-Ⅱmolecules,TLR4receptor and chemokine receptor CCR7on the surface of macrophage stimulated by SjHMGB1group were signifi cantly increased(P<0.01),while TLR2receptor was signi fi cantly decreased(P<0.05).The results of test by ELISA demonstrated that Sj HMGB1could stimulate the secretion of infl amatory factor TNF-α,and fail to promote the release of IL-10on macrophage.The result of test using Griess method showed that SjHMGB1could promote the secretion of NO on macrophage.The data suggest that SjHMGB1could activate mouse macrophages via TLR4and induce macrophages to undergo M1-type infl ammatory polarization.

关 键 词：日本血吸虫 高迁移率族蛋白 巨噬细胞 TLR 细胞因子 

分 类 号：S852.735[农业科学—基础兽医学]