Identification of botanical origin of Chinese unifloral honeys by free amino acid profiles and chemometric methods  

作　　者：Zheng Sun Lingling Zhao Ni Cheng Xiaofeng Xue Liming Wu Jianbin Zheng Wei Cao 

机构地区：[1]Department of Food Science and Engineering, School of Chemical Engineering, Northwest University [2]Institute of Analytical Science, Shaanxi Provincial Key Lab of Electroanalytical Chemistry, Northwest University [3]Institute of Apiculture Research, Chinese Academy of Agricultural Sciences

出　　处：《Journal of Pharmaceutical Analysis》2017年第5期317-323,共7页药物分析学报（英文版）

基　　金：financially supported by the National Natural Science Foundation of China (no. 31272510);the Science Foundation of Xi'an (no. NC1405(1));the Innovation Fund of Graduate Student of Northwest University (no. YZZ13034)

摘　　要：The amino acid contents of five floral sources Chinese honeys(jujube, rape, chaste, acacia, and lungan) were measured using reversed phase high-performance liquid chromatography(RP-HPLC). The results showed that proline was the main amino acid in most of the analyzed samples. Phenylalanine presents at the highest content in chaste honey samples, and the total amino acid contents of chaste honeys were also significantly higher than those of other honey samples. Based on the amino acid contents, honey samples were classified using chemometric methods(cluster analysis(CA), principal component analysis(PCA), and discriminant analysis(DA)). According to the CA results, chaste honeys could be separated from other honeys, while the remaining samples were correctly grouped together when the chaste honey data were excluded. By using DA, the overall correct classification rate reached 100%. The results revealed that amino acid contents could potentially be used as indicators to identify the botanical origin of unifloral honeys.The amino acid contents of five floral sources Chinese honeys(jujube, rape, chaste, acacia, and lungan) were measured using reversed phase high-performance liquid chromatography(RP-HPLC). The results showed that proline was the main amino acid in most of the analyzed samples. Phenylalanine presents at the highest content in chaste honey samples, and the total amino acid contents of chaste honeys were also significantly higher than those of other honey samples. Based on the amino acid contents, honey samples were classified using chemometric methods(cluster analysis(CA), principal component analysis(PCA), and discriminant analysis(DA)). According to the CA results, chaste honeys could be separated from other honeys, while the remaining samples were correctly grouped together when the chaste honey data were excluded. By using DA, the overall correct classification rate reached 100%. The results revealed that amino acid contents could potentially be used as indicators to identify the botanical origin of unifloral honeys.

关 键 词：HONEY Free AMINO ACID CHEMOMETRICS RP-HPLC 

分 类 号：O657.72[理学—分析化学] S896.1[理学—化学]