离子色谱-紫外检测法测定饲料中的胍基乙酸  

Determination of guanidinoacetic acid in feed by ion chromatography coupled with ultraviolet detection

作　　者：孙祥丽 魏妍妍 刘伟国 张丽英[1] SUN Xiangli;WEI Yanyan;LIU Weiguo;ZHANG Liying(Ministry of Agriculture Feed Efficacy and Safety Evaluation Center (Beijing),State Key Laboratory of Animal Nutrition,China Agricultural University,Beijing 100193, China;Beijing Gendone Agricultural Technology Co.,Ltd.,Beijing 100085,China)

机构地区：[1]中国农业大学动物营养学国家重点实验室农业部饲料效价与安全监督检验测试中心(北京),北京100193 [2]北京君德同创农牧科技股份有限公司,北京100085

出　　处：《色谱》2017年第10期1100-1104,共5页Chinese Journal of Chromatography

基　　金：北京市科技计划项目(Z151100001215001)~~

摘　　要：通过对色谱柱、流动相洗脱、样品前处理等条件进行优化,建立了一种检测饲料中胍基乙酸含量的离子色谱法。在甲磺酸线性梯度洗脱条件下,样品经Dionex IonPacTMCS16阳离子交换柱分离,用紫外检测器于200 nm波长处进行检测。在0.5~200 mg/L范围内,中胍基乙酸色谱峰面积与质量浓度呈良好的线性关系(相关系数r2=0.999 9)。配合饲料和浓缩饲料中胍基乙酸的检出限为4.5 mg/kg、定量限为15 mg/kg,复合预混合饲料中胍基乙酸的检出限为9.0 mg/kg、定量限为30 mg/kg。该方法对添加量在15 mg/kg^60 g/kg范围内的禽用配合饲料、猪用配合饲料、浓缩饲料、复合预混合饲料中胍基乙酸的回收率均大于94%。该方法性能指标可满足饲料中胍基乙酸含量的检测需求。A novel ion chromatography method for the determination of guanidinoacetic acid(GAA)in animal feed samples was developed.The optimal detection mode,chromatography column,gradient condition of mobile phases and sample pretreatment conditions were investi-gated.Using a linear gradient of methane sulfonic acid and ultraviolet detector at200nm,the interfering substances in feed samples and GAA could be separated successfully on a Dionex IonPac?CS16cation exchange analysis column.The calibration curves showed a range of linearity between0.5and200mg/L,and a high linear regression coefficient of0.9999was obtained for GAA.The limits of detection and limits of quantification of GAA in formula feed and concentrate feed were4.5and15mg/kg,respectively.The limit of detection and limit of quantification of GAA in compound-premix were9.0and30mg/kg,respectively.The recoveries of this method for GAA were investigated,and the recoveries were all more than94%in formula feed,concentrate feed and compound-premix feed for pig and poultry.This method can meet the requirements of the determination of GAA in feed samples for the feed production enterprise and evaluation center.

关 键 词：离子色谱 紫外检测 阳离子交换 胍基乙酸 饲料 

分 类 号：O658[理学—分析化学]