β-紫罗兰酮脂质体包封率测定方法研究  

Determination of Entrapment Efficiency of Beta-Ionone Liposomes

作　　者：王一涵 徐迎波[2] 宁敏[2] 周志磊[1] 梁蓉 陈羚 徐菲菲[1] 钟芳[1] WANG Yihan;XU Yingbo;NING Ming;ZHOU Zhilei;LIANG Rong;CHEN Ling;XU Feifei;ZHONG Fang(School of Food Science and Technology,Jiangnan University,Wuxi 214122,China;Key Laboratory of Tobacco Chemistry,China Tobacco Anhui Industrial Co.,LTD,Hefei 230088,China;Key Laboratory of Food Colloids and Biotechnology,Ministry of Education,Jiangnan University,Wuxi 214122,China)

机构地区：[1]江南大学食品学院,江苏无锡214122 [2]安徽中烟工业有限责任公司烟草化学安徽省重点实验室,安徽合肥230088 [3]江南大学食品胶体与生物技术教育部重点实验室,江苏无锡214122

出　　处：《食品与生物技术学报》2017年第7期688-691,共4页Journal of Food Science and Biotechnology

基　　金：国家自然科学基金项目(31171686);烟草化学安徽省重点实验室2013年开放课题资助项目(0920140109010)

摘　　要：采用薄膜超声法制备β-紫罗兰酮脂质体,通过反透析法分离脂质体和游离物质,并以高效液相色谱法为分析手段对其包封率进行测定。在选定色谱条件下,辅料不干扰测定,β-紫罗兰酮在5~80 mg/L的质量浓度范围内线性关系良好(R2=0.999 8),日内和日间精密度(RSD)均小于3%;透析平衡时间为12 h,游离物回收率符合要求(99.21%~101.22%),且透析20 h内无渗漏,方法重现性好;使用此法测得β-紫罗兰酮脂质体的平均包封率为(52.76±1.10)%。反透析法简便,准确,经济,适用于β-紫罗兰酮脂质体包封率的测定。A method used for determining the entrapment efficiency of beta-ionone in liposomeswas established.The liposome was prepared by thin film evaporation.The free beta-ionone wasseparated from liposomes by reverse dialysis and quantified with HPLC.The results showed that thecalibrated curve was linear(/JU0.9998)within the range of5?80mg/L for beta-ionone.Intra-dayand inter-day precision(RSD)were both less than3%.The equilibrium time for the dialysis was12h,and the recoveries showed with good reproducibility(99.21%?101,22%),and the leakingpercentage in20h was negligible.The average entrapment efficiency of beta-ionone liposome was52.76±1,10%.The reverse dialysis method was convenient,accurate and economic,suitable fordetermining the encapsulation efficiency of beta-ionone liposomes.

关 键 词：β-紫罗兰酮脂质体 薄膜超声法 包封率 反透析法 

分 类 号：TQ651[化学工程—精细化工]