姜黄素固体脂质纳米粒在大鼠体肠中吸收的研究  

Intestinal Absorption of Curcumin Solid Lipid Nanoparticles in Rats

作　　者：李万玉[1] 晏子俊[1,2] 孙立力[1] 万坤[1] 罗见春 张景勍[1] LI Wanyu;YAN Zijun;SUN Lili;WAN Kun;LUO Jianchun;ZHANG Jingqing(Engineering Research Center in University,Chongqing Medical University,Chongqing 400016,China;Pharmacy of Panzhihua Central Hospital,Panzhihua 617067,China)

机构地区：[1]重庆医科大学药物高校工程研究中心,重庆400016 [2]攀枝花市中心医院药学部,四川攀枝花617067

出　　处：《食品与生物技术学报》2017年第7期733-737,共5页Journal of Food Science and Biotechnology

基　　金：重庆市科委重点项目(CSTC2012JJB10027)

摘　　要：采用乳化-超声法制得姜黄素固体脂质纳米粒(Curcumin solid lipid nanoparticles,CNSLN),比较游离药姜黄素(Curcumin,CRM)和CNSLN的在体肠中吸收情况。选用大鼠在体单向肠灌流模型(Single-pass intestinal perfusion model,SPIP),以紫外分光光度法测定游离CRM和CNSLN通过肠液后CRM的减少量来确定药物的吸收。结果:透射电镜下观察到CNSLN成圆形或椭圆形,平均粒径为(120.7±5.4)nm,平均Zeta电位为(-41.90±1.81)m V,平均包封率为(91.12±0.42)%。游离CRM和CNSLN在十二指肠、空肠、回肠和结肠4段的吸收速率常数(Ka)、有效渗透率(Peff)和百分吸收率(W)均存在显著性差异(P<0.01),CNSLN比游离CRM均提高了2倍以上,且CNSLN的最大吸收部位在结肠。在体肠吸收结果显示,CNSLN的肠吸收比游离CRM高,CNSLN能明显的提高大鼠对游离CRM的肠吸收。To prepare the curcumin solid lipid nanoparticles(CNSLN)with emulsion-ultrasonic and compare the intestinal absorption of free curcumin(CRM)and CNSLN.Single-pass intestinal perfusion(SPIP)model was used to study the intestinal absorption and Ultraviolet spectrometric assay was used to determine the content of CRM.The CNSLN presented as small round or oval under transmission electron microscope(TEM)with the diameter of about(120.7±5.4)nm,zeta potential was(-41.90±1.81)mV,average entrapment efficiency was(91.12±0.42)%.The absorption rate constant(Ka),effective permeability(Peff),percent absorption rate W(%)of CRM and CNSLN in duodenum,jejunum,ileum and colon both were significant difference(P<0.01),and CNSLN was more than2times as much as CRM.The maximum segments of CNSLN absorption was colon.The results indicated that the absorption of CNSLN in rats is better than CRM,and CNSLN could obviously enhance the absorption of CRM in rats.

关 键 词：姜黄素 固体脂质纳米粒 在体单向肠灌流摸型 肠吸收 

分 类 号：R285.5[医药卫生—中药学]