贵州汉族19个STR基因座多态性及法医学应用  

Polymorphisms of 19 STR Loci in Guizhou Han Population and Their Forensic Application

作　　者：赵勤松 任峥[1] 张红玲[1] 戴佳琳[1] 王杰[1] 喻芳 黄江[1] ZHAO Qin-song;REN Zheng;ZHANG Hong-ling;DAI Jia-lin;WANG Jie;YU Fang;HUANG Jiang(Department of Forensic Medicine, Guizhou Medical University, Guiyang 550025, China;Guizhou Province Population and Family Planning Institute of Justice, Guiyang 550004, China)

机构地区：[1]贵州医科大学法医学院,贵州贵阳550025 [2]贵州省人口和计划生育司法鉴定所,贵州贵阳550004

出　　处：《法医学杂志》2017年第4期388-392,共5页Journal of Forensic Medicine

基　　金：贵州省高校工程技术研究中心资助项目(黔科合KY字[2013]114号);贵州省现代法医司法鉴定工程研究中心资助项目(黔发改高技[2016]1345号)

摘　　要：目的调查19个常染色体STR基因座在贵州汉族人群中的等位基因分布,评估其在法医学中的应用价值。方法应用Goldeneye^(TM) DNA身份鉴定系统20A试剂盒,研究贵州520名汉族无关健康个体19个常染色体STR基因座多态性。用310型遗传分析仪进行毛细管电泳,Gene Mapper~ID v3.1进行基因分型。结果 19个常染色体STR基因座的杂合度为0.603 8~0.916 4,个体识别率为0.790 0~0.985 6,非父排除率为0.295 5~0.826 9,多态信息含量为0.553 5~0.908 9,累积个体识别率为1-1.230 0×10^(-22),累积非父排除率为0.999 999 99。贵州汉族和其他五个地域的汉族两两之间等位基因频率比较,仅贵州汉族与山东汉族、辽宁汉族、山西汉族之间存在基因频率差异具有统计学意义。结论 D19S433等19个常染色体STR基因座在贵州汉族人群中具有良好的遗传多态性,对群体遗传学和法医物证学研究有应用价值。Objective To investigate the allelic distribution of19autosomal STR loci in Guizhou Han population,and to estimate the forensic application value.Methods The19autosomal STR loci in520unrelated healthy individuals from Guizhou Han population were studied using GoldeneyeTM20A kit.The310genetic analyzer was used for capillary electrophoresis,and the GeneMapper ID v3.1for genotyping.Results The heterozygosis,the discrimination power,the probability of exclusion,the polymorphism information content,the cumulative discrimination power and the cumulative probability of exclusion of the19STR loci were0.6038-0.9164,0.7900-0.9856,0.2955-0.8269,0.5535-0.9089,1-1.2300×10-22and0.99999999,respectively.Compared with other five Han populations in pairwise allelic frequencies,Guizhou Han only had significant differences with Shandong Han,Liaoning Han and Shanxi Han.Conclusion The19autosomal STR loci such as D19S433have a highly genetic polymorphic in Guizhou Han population,which have application values in the researches of population genetics and forensic genetics.

关 键 词：法医遗传学 多态现象 遗传 短串联重复序列 GoldeneyeTM DNA身份鉴定系统20A试剂盒 汉族 贵州 

分 类 号：DF795.2[医药卫生—法医学]