Antioxidant Response of Ridgetail White Prawn Exopalaemon carinicauda to Harmful Dinoflagellate Prorocentrum minimum Exposure and Its Histological Change  

作　　者：MU Cuimin REN Xianyun GE Qianqian WANG Jiajia LI Jian 

机构地区：[1]Key Laboratory for Sustainable Utilization of Marine Fisheries Resources of Ministry of Agriculture,Yellow Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences [2]Function Laboratory for Marine Fisheries Science and Food Production Processes,Qingdao National Laboratory for Marine Science and Technology

出　　处：《Journal of Ocean University of China》2017年第2期285-293,共9页中国海洋大学学报（英文版）

基　　金：supported by the Special Fund for Agro-scientific Research in the Public Interest of China(No.2060302201515013);the Scientific and Technological Innovation Project Financially Supported by Qingdao National Laboratory for Marine Science and Technology(No.2015ASKJ02)

摘　　要：The dinoflagellate Prorocentrum minimum,one of the most widespread red tide causing species,affects marine aquaculture and ecosystems worldwide.In this study,ridgetail white prawn Exopalaemon carinicauda were exposed to P.minimum cells(5 × 104 cells mL-1) to investigate its harmful effects on the shrimp.Antioxidant activities and histological changes were used as indicators of health status of the shrimp.In 72 hours,the mortality of E.carinicauda was not affected,but its antioxidant response and histology were statistically different from those of control.Elevated superoxide dismutase(SOD) and glutathione peroxidase(GPX) activities and depressed catalase(CAT) activity were observed in gill;while increased SOD,glutathione S-transferase(GST),CAT activities and modulated GPX activity were observed in hepatopancreas.Thus,antioxidant activities in gill and hepatopancreas seem to respond differentially to harmful alga exposure.Increased malondialdehyde(MDA) content in early a few hours indicates the damage of the antioxidant defense system.Although MDA content recovered to a low level thereafter,a series of histological abnormalities including accumulation or infiltration of hemocytes,tissue lesions and necrosis were discovered in gill and hepatopancreas.Exposure to P.minimum induced sublethal effects on E.carinicauda,including temporary oxidative damage and histological injury.The dinoflagellate Prorocentrum minimum, one of the most widespread red tide causing species, affects marine aquaculture and ecosystems worldwide. In this study, ridgetail white prawn Exopalaemon carinicauda were exposed to P. minimum cells (5 × 10⁴ cells mL⁻¹) to investigate its harmful effects on the shrimp. Antioxidant activities and histological changes were used as indicators of health status of the shrimp. In 72 hours, the mortality of E. carinicauda was not affected, but its antioxidant response and histology were statistically different from those of control. Elevated superoxide dismutase (SOD) and glutathione peroxidase (GPX) activities and depressed catalase (CAT) activity were observed in gill; while increased SOD, glutathione S-transferase (GST), CAT activities and modulated GPX activity were observed in hepatopancreas. Thus, antioxidant activities in gill and hepatopancreas seem to respond differentially to harmful alga exposure. Increased malondialdehyde (MDA) content in early a few hours indicates the damage of the antioxidant defense system. Although MDA content recovered to a low level thereafter, a series of histological abnormalities including accumulation or infiltration of hemocytes, tissue lesions and necrosis were discovered in gill and hepatopancreas. Exposure to P. minimum induced sublethal effects on E. carinicauda, including temporary oxidative damage and histological injury.

关 键 词：PROROCENTRUM minimum EXOPALAEMON carinicauda ANTIOXIDANT response HISTOLOGICAL changes 

分 类 号：S945.4[农业科学—水产养殖]