含锌镁合金对人成骨肉瘤U2OS细胞株体外增殖的影响  

Effects of zinc-containing magnesium alloys on proliferation of human osteosarcoma U2OS cells in vitro

作　　者：赵小魁 何保华 邵楠 张胜国 杨万石 Zhao Xiao-kui;He Bao-hua;Shao Nan;Zhang Sheng-guo;Yang Wan-shi(Department of Orthopedics, China Meitan General Hospital, Beijing 100028, China)

机构地区：[1]煤炭总医院骨科,北京市100028

出　　处：《中国组织工程研究》2017年第30期4769-4774,共6页Chinese Journal of Tissue Engineering Research

摘　　要：背景:合金化可提高纯镁的抗腐蚀性能,延缓其降解速率;大量文献表明锌具有良好的抗肿瘤效果。目的:评价含锌镁合金对人成骨肉瘤U2OS细胞体外增殖及凋亡的影响。方法:检测纯镁及不同含锌量镁合金(质量分数2%、4%、6%)在Hank’s液中的氢气释放速率。取人成骨肉瘤U2OS细胞(或MC3T3-E1细胞),分别与不同含锌量镁合金浸提液、纯镁浸提液及钛合金浸提液共培养1,3,5 d,MTT法检测细胞增殖。取人成骨肉瘤U2OS细胞,分别与不同含锌量镁合金浸提液、纯镁浸提液及钛合金浸提液共培养24 h,流式细胞仪分析细胞凋亡,Western Blotting检测凋亡相关蛋白的表达水平。结果与结论:①在初始的100 h内,纯镁组氢气释放速率明显高于含锌镁合金组;在不同含锌量镁合金组中,以含锌量4%镁合金组氢气释放速率最慢;②不同含锌量镁合金均可明显抑制人成骨肉瘤U2OS细胞的增殖,且与锌含量呈正相关;不同含锌量镁合金对MC3T3-E1细胞的毒性在0至1级之间;③不同含锌量的镁合金可明显促进人成骨肉瘤U2OS细胞的凋亡,且与锌含量呈正相关;④不同含锌量镁合金可上调人成骨肉瘤U2OS细胞p53、Bax蛋白表达,下调Bcl-2蛋白表达,改变Bcl-2/Bax比例,其中以含锌量4%镁合金效果最显著;⑤结果表明:含锌镁合金可抑制人成骨肉瘤U2OS细胞的体外增殖,诱导其凋亡。BACKGROUND:Alloying can improve the corrosion resistance and slow the degradation of pure magnesium.In addition,increasing studies have shown that zinc has good antitumor effect.OBJECTIVE:To evaluate the effect of zinc-containing magnesium alloy on the proliferation and apoptosis of human osteosarcoma U2OS cells in vitro.METHODS:The corrosion resistance of pure magnesium and different zinc-containing(2%,4%,6%)magnesium alloys were observed and compared by the hydrogen release assay in the Hank’s solution.MTT assay was used to examine the effects of different zinc-containing magnesium alloy extractions on the proliferation of U2OS cells(or MC3T3-E1cells)after co-culture of1,3,5days.After24-hour co-culture with pure magnesium,different zinc-containing magnesium alloys and titanium alloy extractions,the apoptotic rates of U2OS cells were analyzed by flow cytometry,and the expression of apoptosis-related proteins were detected by western blot assay.RESULTS AND CONCLUSION:The corrosion resistance of pure magnesium was improved after addition of Zn within the initial100hours,and the magnesium alloy containing4%zinc exhibited the optimal corrosion resistance.Different zinc-containing magnesium alloy extractions obviously inhibited the U2OS proliferation in a zinc level-depended manner,and the cytotoxicity of different zinc-containing magnesium alloy extractions to MC3T3-E1was graded0-1.Different zinc-containing magnesium alloy extractions could also induce obvious apoptosis in U2OS cells in a zinc level-depended manner.Different zinc-containing magnesium alloy extractions,especially the magnesium alloy containing4%zinc,up-regulated the expression of p53and Bax proteins and down-regulated the expression of Bcl-2protein in U2OS cells,leading to the disorder of Bcl-2/Bax.These findings suggest that different zinc-containing magnesium alloy extractions can inhibit the proliferation and induce apoptosis of U2OS cells in vitro.

关 键 词：生物材料 骨生物材料 镁锌合金 人成骨肉瘤U2OS细胞 细胞增殖 细胞凋亡 

分 类 号：R318[医药卫生—生物医学工程]