骨髓间充质干细胞复合双相磷酸钙陶瓷对幼年大鼠软骨损伤修复的影响  

Bone marrow mesenchymal stem cells/biphasic calcium phosphate ceramics for cartilage repair in juvenile rats

作　　者：李国威[1] 郭远清[1] 陈涛[1] 张奎渤[1] 于兵[1] 张大卫[1] 张荣凯[1] Li Guo-wei;Guo Yuan-qing;Chen Tao;Zhang Kui-bo;Yu Bing;Zhang Da-wei;Zhang Rong-kai(Department of Spinal Surgery, Fifth Affiliated Hospital of Sun Yat-sen University, Zhuhai 519000, Guangdong Province, China)

机构地区：[1]中山大学附属第五医院脊柱外科,广东省珠海市519000

出　　处：《中国组织工程研究》2017年第30期4781-4786,共6页Chinese Journal of Tissue Engineering Research

摘　　要：背景:小儿股骨头坏死症的发病机制与压力负荷和炎症导致的髋关节软骨损伤有关。目的:观察骨髓间充质干细胞复合双相磷酸钙陶瓷对幼年大鼠软骨损伤修复的影响。方法:将30只1周龄SD雄性大鼠随机分为3组,即空白组、对照组和观察组。其中,空白组不进行任何处理,对照组和观察组采用改良Hulth方法制备幼年大鼠髋关节软骨损伤模型,观察组大鼠植入骨髓间充质干细胞复合双相磷酸钙陶瓷,对照组大鼠植入骨髓间充质干细胞复合羟基磷灰石陶瓷。植入后4周,观察各组关节软骨的病理学变化,采用MTT法和流式细胞仪测定各组软骨细胞的增殖和凋亡情况。结果与结论:①空白组关节软骨平滑、完整;对照组关节软骨损害明显,关节软骨表面破裂、缺损、凹凸不平,软骨四层结构不清晰;观察组关节软骨损伤得到一定程度的修复;②在相同观察时间点,观察组的细胞活性较对照组显著升高(均P<0.05),凋亡细胞比例显著降低(均P<0.05);③结果表明,骨髓间充质干细胞复合双相磷酸钙陶瓷能促进幼年大鼠软骨损伤的修复。BACKGROUND:The pathogenesis of pediatric femoral head necrosis is associated with cartilage injury of the hip joint induced by stress and inflammation.OBJECTIVE:To observe the effect of bone marrow mesenchymal stem cells(BMSCs)/biphasic calcium phosphate ceramics(B-CPC)on cartilage repair in juvenile rats.METHODS:Thirty male Sprague-Dawley rats,aged1week,were randomized into three groups.No intervention was done in blank group.A juvenile rat model of articular cartilage injury was made using improved Hulth’s method in control and observational groups,followed by implantation of BMSCs/hydroxyapatite and BMSCs/B-CPC,respectively.Four weeks later,the rat articular cartilage was observed pathologically,and MTT and flow cytometry were employed to detect chondrocyte proliferation and apoptosis,respectively.RESULTS AND CONCLUSION:The articular cartilage of the rats in the blank group was smooth and complete.In the control group,articular cartilage damage was obvious,presenting with rupture,defect and irregularity of the articular cartilage surface,as well as unclear four-layer structure of the cartilage.In the observational group,articular cartilage injury was repaired to some extent.At the same observation time,the cell viability was significantly increased in the observational group compared with the control group(all P<0.05),and the proportion of apoptotic cells was significantly decreased(all P<0.05).To conclude,BMSCs/B-CPC composite can promote the cartilage repair in juvenile rats.

关 键 词：生物材料 软骨生物材料 骨髓间充质干细胞 软骨细胞 细胞凋亡 双相磷酸钙陶瓷 小儿股骨头坏死症 髋关节软骨损伤 

分 类 号：R318[医药卫生—生物医学工程]