胶原蛋白海绵对肝脏创伤止血的效果  

Hemostatic effect of collagen sponge on liver trauma

作　　者：张自强[1] 张以河[1] 祖耀先 安琪[1] 徐荣荣 Zhang Zi-qiang;Zhang Yi-he;Zu Yao-xian;An Qi;Xu Rong-rong(Beijing Key Laboratory of Nonmetallic Minerals and Solid Waste Reuse, School of Materials Science and Technology, China University of Geosciences,Beijing 102200, China;Beijing Red Cross Emergency Rescue Center, Beijing 102200, China;PASHIONBIO, Beijing 102200, China)

机构地区：[1]非金属矿物与固废资源材料化利用北京市重点实验室,中国地质大学(北京)材料科学与工程学院,北京市102200 [2]北京市红十字会急诊抢救中心,北京市102200 [3]北京湃生生物科技有限公司,北京市102200

出　　处：《中国组织工程研究》2017年第30期4874-4879,共6页Chinese Journal of Tissue Engineering Research

摘　　要：背景:艾瑞金胶原蛋白海绵理化性能稳定,并已通过国家食品药品监督管理局的理化性能和生物相容性评价检测。目的:观察艾瑞金胶原蛋白海绵的止血效果。方法:取21只SD大鼠,建立肝脏出血创面,随机分3组干预:在实验组(n=7)肝脏创口内部植入艾瑞金胶原蛋白海绵,同时在肝脏切口表面外敷艾瑞金胶原蛋白海绵;在阳性对照组(n=7)肝脏创口内部植入医用胶原蛋白海绵,同时在肝脏切口表面外敷医用金胶原蛋白海绵;在空白对照组(n=7)肝脏切口表面外敷医用纱布,记录出血量及止血时间,干预后7,14,28 d进行肝脏创面组织学观察。结果与结论:①出血量及止血时间:3组出血量比较无差异;实验组、阳性对照组止血时间与空白对照组比较差异无显著性意义,实验组止血时间短于阳性对照组(P≤0.05);②肝脏创面组织学观察:实验组干预后7 d胶原材料被纤维结缔组织完全包裹,炎性细胞浸润以中性粒细胞为主,胶原材料开始降解,周边结缔组织内有新生毛细血管;干预后14 d,包裹胶原材料的纤维结缔组织明显增厚,中性粒细胞减少,巨噬细胞增多;干预后28 d,胶原材料完全降解,大部肝组织恢复正常,部分肝组织旁的炎性结缔组织中可看到巨噬细胞、单核细胞、成纤维细胞和毛细血管。阳性对照组情况类似于实验组。空白对照组干预后14 d创伤处结缔组织明显,肝窦含有红细胞,偶见肝组织内出血,空泡变性;干预后28 d,创口处具有较厚结缔组织,肝窦含有红细胞,被复肝星状细胞;③结果表明:艾瑞金胶原蛋白海绵对肝脏创伤止血效果明显,组织相容性好。BACKGROUND:iRegene collagen sponge exhibits stable physical and chemical properties,and has passed the test by the State Food and Drug Administration of China.OBJECTIVE:To study the hemostatic effect and the biocompatibility of the iRegene collagen sponge on a liver wound by means of rat models.METHODS:Liver trauma bleeding models were made in21Sprague-Dawley rats.These model rats were randomized into three groups(n=7per group):experimental group with implantation and external application of iRegene collagen sponge;positive control group with implantation of medical collagen sponge and external application of iRegene collagen sponge;blank control group with external application of medical gauze.The bleeding time and amount on the liver wounds were observed.Histological observation on the liver wound was performed at7,14,28days after intervention.RESULTS AND CONCLUSION:The bleeding time was shorter in the experimental group than the positive control group(P≤0.05).Beyond that,there was no difference in the bleeding amount and time among the three groups.Histological findings on the liver wound showed that the iRegene collagen sponge in the experimental group was completely wrapped with fibrous connective tissues and began to degrade at7days after intervention,the Inflammatory cell infiltration mainly occurred in neutrophils,and new capillaries were observed in peripheral connective tissues;at14days after intervention,the fibrous connective tissues became remarkably thickened,the number of neurophils was reduced,and the number of macrophages was increased;at28days after intervention,the iRegene collagen sponge degraded completely,most of the liver tissues recovered,and there were macrophages,monocytes,fibroblasts and capillaries in the inflammatory connective tissues adjacent to a part of liver tissues.Similar findings were observed in the positive control group.In the blank control group,there were obvious connective tissues on the wound and red blood cells in the liver sinus,and occasionally liver tissue bleedin

关 键 词：生物材料 材料相容性 胶原蛋白海绵 止血 肝脏创伤 组织相容性 

分 类 号：R318[医药卫生—生物医学工程]