云南烤烟根际土壤PGPR菌株的筛选与鉴定  

Isolation and Identification of PGPR Strains from Rhizosphere Soil of Yunnan Flue-cured Tobacco

作　　者：黄智华 崔永和 计思贵 苏友波[2] 张立猛 HUANG Zhihua;CUI Yonghe;JI Sigui;SU Youbo;ZHANG Limeng(Yunnan Tobacco Company, Yuxi Branch, Biological Control for Tobacco Diseases and Insect Pests Engineering Research Center of China Tobacco, Yuxi, Yunnan 653100, China;College of Resource and Environment, Yunnan Agricultural University, Kunming 650201, China)

机构地区：[1]云南省烟草公司玉溪市公司,烟草行业病虫害生物防治工程研究中心,云南玉溪653100 [2]云南农业大学资源与环境学院,昆明650201

出　　处：《中国烟草科学》2017年第5期18-23,共6页Chinese Tobacco Science

基　　金：云南省烟草公司科技计划项目“烤烟根际解磷解钾促生菌PGPR的筛选及应用推广”(2012YN43),“密集种植烟区土壤保育及烟叶质量提升技术研究与应用”(2017YN17)

摘　　要：为了利用本地PGPR菌株改善云南植烟土壤质量,提高磷、钾等元素利用率,促进烤烟生长,在云南主要植烟区采集烤烟根际土壤样品31个,对根际土壤中的微生物进行分离,筛选和鉴定,共获得解磷、解钾或促生菌共773株,进一步筛选获得到了10株具有较强解磷、解钾或IAA促生作用的菌株。经鉴定发现10个菌株分别属于Bacillus(芽孢杆菌属)、Paenibacillus(类芽孢杆菌属)、Providencia(普罗威登斯菌属)。筛选出的PGPR为微生物肥料的开发提供了优质的菌种资源,进一步研究将关注PGPR菌株的混配以及在土壤中的定殖。To improve the quality of tobacco-planting soil,the utilization rate of fertilizer and finally the quality of tobacco in Yunnan Province by local PGPR,rhizosphere soil samples were collected from the main tobacco-planting fields of Yunnan Province.Rhizosphere bacteria were isolated,screened and identified from soil samples.Capacity of potassium-dissolving,phosphate solubilizing and IAA secreting of bacteria were focused.Morphological observation,biochemical analysis and16s rDNA sequencing were used to identify the species of PGPR candidates.In result,31samples of rhizosphere soil were collected from cities of Yuxi,Kunming,Qujing,Honghe,Chuxiong,Dali,and773strains of PGPR were isolated from these soil samples.Ten effective strains were obtained after further screening.The strains belongs to Bacillus,Paenibacillus and Providencia.This research provides resource of excellent PGPR strains for further development of microbial fertilizer.Further works will focus on the mixture andn colonization research of these PGPR candidates.

关 键 词：烤烟 PGPR 解磷 解钾 IAA促生 

分 类 号：S572[农业科学—烟草工业]