脑海马神经元接受化疗与未化疗的差异  

Neurons in the hippocampus of chemobrain versus non-chemotherapy brain

作　　者：李建平[1,2] 卢巍[1] 杨琳[1] 谢密新[3] 贺旭 潘爱华 Li Jian-ping;Lu Wei;Yang Lin;Xie Mi-xin;He Xu;Pan Ai-hua

机构地区：[1]遵义医学院珠海校区人体解剖学教研室,广东省珠海市519041 [2]中南大学湘雅医学院人体解剖与神经生物学系,湖南省长沙市410013 [3]益阳医学高等专科学校人体解剖学教研室,湖南省益阳市413000 [4]武汉大学医学职业技术学院,湖北省武汉市430060

出　　处：《中国组织工程研究》2017年第28期4523-4528,共6页Chinese Journal of Tissue Engineering Research

基　　金：国家自然科学基金(61650305);遵义医学院硕士启动基金(F-77)~~

摘　　要：背景:研究认为化疗药物能穿过血脑屏障引起神经毒性作用而损伤神经细胞。目的:观察化疗脑海马神经元的表达情况及形态的变化,探讨化疗脑对海马神经再生的影响的机制。方法:收集恶性肿瘤化疗人脑标本(<60岁,n=3;>60岁,n=3)和未化疗人脑标本(<60岁,n=3;>60岁,n=3),免疫组织化学检测海马CA1、CA2和CA3区特异抗体阳性神经元的形态及数量的变化情况。结果与结论:(1)不同年龄组免疫组织化学染色结果显示,与未化疗脑相比,化疗脑海马CA1、CA2和CA3区:Neu N^+细胞、DCX^+细胞、PV^+细胞阳性数量明显降低,并且PV^+细胞胞体较小,突起减少并缩短;细胞表达明显减少;(2)免疫荧光染色结果显示,<60岁化疗脑NeuN阳性神经元和DCX阳性神经元共表达数量显著较未化疗脑低,而>60岁化疗脑与未化疗脑无明显差异;(3)结果表明,药物化疗使海马未成熟神经元的表达降低;药物化疗使海马成熟神经元的数量降低;药物化疗使PV神经元形态发生改变,细胞数量降低;药物化疗降低海马未成熟神经元向成熟神经元的转化。BACKGROUND:Chemotherapy drugs have been shown to penetrate the blood-brain barrier and cause neurotoxicity,further damaging nerve cells.OBEJCTIVE:To investigate the changes in the expression and morphology of hippocampal neurons in chemobrain,and to investigate the underlying mechanism of nerve regeneration in chemobrain.METHODS:The brain specimens were obtained from the six patients undergoing cancer chemotherapy and six patients without chemotherapy,and were then subdivided into two groups:<60years and>60years groups(n=3per group).Immunofluorescence staining with specific antibodies was used to detect the changes in the morphology and number of neurons in the hippocampus CA1,CA2and CA3.RESULTS AND CONCLUSION:The number of neurons positive for NeuN,DCX and PV in the hippocampal CA1,CA2and CA3in the chemotherapy group was significantly lower than that in the non-chemotherapy group.Moreover,the neurons positive for PV were smaller,with less and shorter nervous processes.Immunofluorescence staining findings showed that the<60years old patients in the chemotherapy group had less neurons positive for NeuN and DCX than that in the non-chemotherapy;for>60years old patients,there was no significant difference between two groups.These findings suggest that chemotherapy can downregulate the expression of immature neurons in the hippocampus and reduce the number of neurons in the hippocampus.Moreover,chemotherapy can change the morphology and reduce the number of PV neurons.

关 键 词：组织构建 组织工程 化疗脑 海马 DCX PV NEUN 国家自然科学基金 

分 类 号：R318[医药卫生—生物医学工程]