Wnt/β-catenin信号通路对牙根发育的影响  

Role of Wnt/beta-catenin signaling pathway during tooth root development

作　　者：何梅 吴家媛[1,2,3] He Mei;Wu Jia-yuan(Zunyi Medical University, Zunyi 563000, Guizhou Province, China;Stomatological Hospital Affiliated to Zunyi Medical University, Zunyi 563000, Guizhou Province, China;Special Key Laboratory of Oral Diseases Research of Universities in Guizhou Province, Zunyi 563000, Guizhou Province, China)

机构地区：[1]遵义医学院,贵州省遵义市563000 [2]遵义医学院附属口腔医院,贵州省遵义市563000 [3]贵州省高等学校口腔疾病研究特色重点实验室,贵州省遵义市563000

出　　处：《中国组织工程研究》2017年第28期4556-4562,共7页Chinese Journal of Tissue Engineering Research

基　　金：国家自然科学基金资助项目(81460102)~~

摘　　要：背景:Wnt/β-catenin信号通路在牙齿发育过程中发挥重要的作用,其对牙根发育至关重要,参与牙根发育的启动,调控Hertwig’s上皮根鞘的形成,根部成牙本质细胞和成牙骨质细胞的增殖与分化等重要过程。目的:文章对Wnt/β-catenin信号通路在牙根发育过程中的作用及影响进行综述。方法:通过检索CNKI,PubMed等数据库,分别以"Wnt/β-catenin信号通路,牙胚发育,牙齿,成牙本质细胞,成牙骨质细胞"的中文检索词和"Wnt/β-catenin signaling pathway,signaling pathway,tooth,root,Hertwig’s epithelial root sheath,odontoblasts,cementoblast,SCAP"的英文检索词进行检索,根据文献纳入标准和排除标准,提取与Wnt/β-catenin信号通路调控牙根发育过程成牙本质细胞和成牙骨质细胞增殖、分化、Hertwig’s上皮根鞘形成密切相关的文献41篇,利用以上文献进行综述。结果与结论:在牙根发育过程中,Wnt/β-catenin信号通路可以调控Hertwig’s上皮根鞘的完整性,该信号通路的抑制将会导致Hertwig’s上皮根鞘形成中断,继而诱导根部成牙本质细胞和成牙骨质细胞分化失败。该信号通路的缺失或过表达后,成牙本质细胞的增殖和分化受到抑制,根部牙本质形成障碍;而β-catenin信号对牙骨质分化调控结果可受细胞的来源或发育阶段,周围所处的微环境及Wnt信号相关激活剂和抑制剂浓度影响,出现对成牙骨质细胞不同程度的促进和抑制作用。BACKGROUND:Wnt/β-catenin signaling pathway plays an important role in tooth development and especially in the root development,including the initiation of root development,Hertwig’s epithelial root sheath formation,root odontoblast and cementoblast proliferation and differentiation process.OBJECTIVE:To explain the effect of Wnt/β-catenin signaling pathway on tooth root development.METHODS:CNKI and PubMed databases were searched for the literatures concerning odontoblast and cementoblast proliferation and differentiation as well as Hertwig’s epithelial root sheath formation mediated by Wnt/β-catenin signaling pathway during root development.Finally41eligible articles were enrolled according to the inclusion and exclusion criteria.RESULTS AND CONCLUSION:During the tooth root development,Wnt/β-catenin signaling pathway can regulate Hertwig’s epithelial root sheath integrity,so the inhibition of signaling pathway will block Hertwig’s epithelial root sheath formation,further inducing the failure in root odontoblasts and cementoblast differentiation.Loss or overexpression of the signal transduction pathway will inhibit the proliferation and differentiation of odontoblasts,and appear with root dentin formation disorder.Effect ofβ-catenin signal on cementum differentiation can be regulated by cell origin or developmental stage,surrounding microenvironment and Wnt-related activator and inhibitor concentration,thereby promoting or inhibiting cementoblasts to different exents.

关 键 词：组织构建 组织工程 WNT/Β-CATENIN信号通路 Wnt蛋白 牙胚发育:Hertwig’s上皮根鞘 成牙本质细胞 成牙骨质细胞 牙囊细胞:根尖牙乳头干细胞:牙根发育:牙再生组织工程 国家自然科学基金 

分 类 号：R318[医药卫生—生物医学工程]