中山杉微体快繁技术研究  

Micro-propagation of Taxodium hybrid 'zhongshanshan'

作　　者：郝树芹[1,2] 朱坤 陈昆 HAO Shu-qin;ZHU Kun;CHEN Kun(Laiwu Vocational and Technical College, Laiwu Shandong 271100, P.R.China;Shandong Agriculture and Engineering University, Ji5 nan Shandong 250100, P.R.China;Shandong Vocational College of Science and Technology, Weifang Shandong 261053, P.R.China;Shangqiu Academy of Agriculture and Forestry Sciences, Shangqiu He5 nan 476000, P.R.China)

机构地区：[1]莱芜职业技术学院,山东莱芜271100 [2]山东农业工程学院,山东济南250100 [3]山东科技职业学院,山东潍坊261053 [4]商丘市农林科学院,河南商丘476000

出　　处：《西部林业科学》2017年第5期135-138,共4页Journal of West China Forestry Science

基　　金：莱芜职业技术学院博士基金项目

摘　　要：以优良无性系中山杉301茎段为外植体,通过在MS或1/2MS培养基中添加不同浓度和比例的NAA、6-BA、IBA、KT对其进行芽诱导分化、增殖培养和生根诱导,以提升中山杉微体快繁技术研究水平。研究结果表明:以中山杉301带腋芽幼嫩枝条为外植体可以实现微体快繁。MS培养基中NAA含量较高时可以增加萌芽数量,6-BA含量过高对萌芽有抑制作用;诱导分化培养基配方以MS+NAA 0.2mg/L+6-BA 0.4mg/L效果最佳。增殖培养配方以MS+NAA 0.3mg/L+6-BA 0.4mg/L效果最佳,丛芽分化良好,增殖倍数3.8。相同浓度的IBA和NAA以1/2MS生根诱导数量远远高于MS培养基,生根诱导配方以1/2MS+IBA 0.3mg/L+NAA 0.2mg/L效果最好,较相同激素条件下的MS培养基生根率提高8%。To explore the micro-propagation,the stems of Taxodium hybrid‘zhongshanshan’301superior clones were applied as explants to conduct experiments of the bud differentiation,enrichment culture and root induction in MS and1/2MS medium with different concentration and proportion of NAA,6-BA,IBA and KT.The results showed that:The explant which was stem piece with bud of Taxodium hybrid‘zhongshanshan’301presented well for propagation.There were more lateral buds if more NAA was added into MS medium,and the content of6-BA was too high to inhibit germination.Optimum directions for culture media for inducement and differentiation was MS+NAA0.2mg/L+6-BA0.4mg/L.Optimum directions for culture media for proliferation was MS+NAA0.3mg/L+6-BA0.4mg/L,and bud differentiation was good,multiplication rate was3.8.Optimum directions for culture media for root inducing was1/2MS+IBA0.3mg/L+NAA0.2mg/L,and the number of IBA and NAA on rooting was significantly higher in1/2MS medium than that in MS medium,rooting rate increased by8%compared to the same hormone under the condition of MS culture,significant difference.

关 键 词：中山杉 微体快繁 诱导分化 增殖培养 生根诱导 

分 类 号：S791[农业科学—林木遗传育种]