两种方法诱导人iPSCs分化为心肌细胞的对比  

Comparison of Two Techniques for the Induction of Human Pluripotent Stem Cell Differentiation towards Cardiomyocytes

作　　者：戴青原[1] 孙贵虎 徐静舒[2] 谭小兵[2] DAI Qing-yuan;SUN Gui-hu;XU Jing-shu;TAN Xiao-bing(Dept. of Cardiology,The First Affiliated Hospital of Kunming Medical University, Kunming Yunnan 650032;Dept. of Cariology and Endodontics,The First People's Hospital of Yunnan Province,Kunming Yunnan 650032,China)

机构地区：[1]昆明医科大学第一附属医院心内科,云南昆明650032 [2]云南省第一人民医院口腔内科,云南昆明650032

出　　处：《昆明医科大学学报》2017年第5期21-25,共5页Journal of Kunming Medical University

基　　金：国家自然科学基金资助项目(81360161);云南省教育厅科学研究基金资助项目(2015Y153)

摘　　要：目的对比研究两种方法诱导人牙源性i PSCs分化为心肌细胞的效率.方法 EB法和单层培养法将人DPSCs-i PSCs诱导分化为心肌细胞,比较心肌细胞的生物学特征、分化时间,流式细胞仪检测心肌细胞的特异性标记物TNNT2阳性率.结果 2种方法得到的心肌细胞具有自我搏动的特性,分化时间:EB法(23.33±1.45)d多于单层培养法(10.33±0.88)d,P<0.05,分化效率:EB法(10.0±1.73)%低于单层培养法(88.67±2.60)%,P<0.05.结论单层培养法诱导时间短、分化效率高,是人牙源性i PSCs分化为心肌细胞的适合方法.Objective To compare two techniques for the induction of human dental induced pluripotent stemcells(iPSCs)differentiation towards cardiomyocytes.Methods Human DPSCs-iPSCs were induced tocardiomyocytes using embryoid body(EB)formation and monolayer culture methods.Basic biological behavior,induction time and TNNT2(Troponin T type2)expression via flow cytometry were compared.Results Bothderived cardiomyocytes showed specific spontaneous contracting.Induction time for EB was23.3±1.45d,whichwas longer than that of monolayer method(10.33±0.88d)(P<0.05),while the efficiency(10.0±1.73%)was lower than that of monolayer method(88.67±2.60%)(P<0.05).Conclusions Monolayer culture is anoptimal method for the induction of human DPSCs-iPSCs into cardiomyocytes with short time and high efficiency.

关 键 词：人诱导多潜能干细胞 分化 心肌细胞 

分 类 号：R541.4[医药卫生—心血管疾病]