四聚乙醛在亚急性中毒大鼠部分组织中的分布  

Distribution of Metaldehyde in Some Tissues of Subacutely Poisoned Rats

作　　者：李鸿 郑洪富 解润芳 李树华 张瑞林 王蕊 李慧方洁 LI Hong;ZHENG Hong-fu;XIE Run-fang;LI Shu-hua;ZHANG Rui-lin;WANG Rui;LI Hui-fang jie(Criminal Investigation Branch of the Qujing Public Security Bureau,Yunnan Qujing 655000;School of Forensic Medicine,Kunming Medical University,Yunnan Kunming 650500,China)

机构地区：[1]曲靖市公安局刑侦支队,云南曲靖655000 [2]昆明医科大学法医学院,云南昆明650500

出　　处：《昆明医科大学学报》2017年第7期7-11,共5页Journal of Kunming Medical University

基　　金：国家自然科学基金资助项目(81460213);云南省科技厅-昆明医科大学应用基础研究联合专项基金资助项目(2014FB007)

摘　　要：目的建立生物检材中四聚乙醛气相色谱/质谱以及气相色谱-FID的分析方法,考察四聚乙醛在亚急性中毒大鼠部分组织中的分布情况.方法 SD大鼠口服给药四聚乙醛(40 mg/kg,140 mg/kg,280 mg/kg)1周后麻醉处死,心血、肝脏、胃组织、肾脏提取后检测,对照组大鼠以生理盐水口服给药,各对应组织器官样品为空白对照样.采用二氯甲烷液-液萃取,气相色谱/质谱进行定性分析、气相色谱-FID定量分析生物检材中的四聚乙醛的浓度.结果建立了生物检材中四聚乙醛的GC/MS及GC-FID分析方法.GC/MS分析方法最低检出限为0.1 ng,GC-FID分析方法在0.01~0.1 mg/mL范围线性良好(R=0.9992),平均回收率大于75%.高、中、低剂量染毒后四聚乙醛在胃组织中的浓度最高,而常见药物代谢的肝脏组织中浓度最低.结论四聚乙醛在亚急性中毒大鼠体的含量分布情况为:胃组织>心血>肾脏>肝脏,该方法操作简便快速,检测灵敏度高,干扰小,适用于体内四聚乙醛含量的测定.Objective To develop the method for the determination of metaldehyde in biological material by gas chromatography/mass spectrometry(GC/MS)and GC-FID and to investigate the distribution of metaldehyde in main tissues of subacutely poisoned rats.Methods SD rats were given the oral administration of metaldehyde(40mg/kg,140mg/kg,280mg/kg)and executed by hypoxia after one week.Cardiac blood,liver,stomach and kidney were taken out and the contents of metaldehyde were detected.The rats in control group were given the oral administration of physiologic saline,and their blood and tissues were taken out correspondingly to be used as blank.The blood and tissues samples were extracted by dichloromethane.The metaldehyde was qualitatively analyzed by GC/MS and quantitatively analyzed by GC-FID.Results A GC/MS and GC-FID method for the determination of metaldehyde in biological samples was established.The limit of detection of GC/MS was0.1ng while the GC-FID method displayed a satisfactory linearity within the concentration ranges of0.01～0.1mg/mL(R=0.9992).The average recovery was over75%.Conclusion The order of metaldehyde content in blood and tissues is:gastric tissues>cardiac blood>kidney>liver.This method is simple,quick,accurate,replicated and suitable for the determination of metaldehyde in biological samples.

关 键 词：四聚乙醛 生物检材 气相色谱/质谱法 气相色谱法 体内分布 

分 类 号：R991[医药卫生—毒理学]