Caspase-9在髁突软骨及长骨生长板软骨中表达变化的对比  

Comparison of Caspase-9 Expression in Condylar Cartilage and Growth Plate Cartilage in the Development Process

作　　者：何影 毛瑞[2] 刘绍梅 李怡莹 李松[2] HE Ying;MAO Rui;LIU Shao-Mei;LI Yi-ying;LI Song(Dept. of Stomatology,Yunnan General Hospital of Armed Police Forces,Kunming Yunnan 650111;Kunming Medical University,Kunming Yunnan 650500,China)

机构地区：[1]武警云南总队医院口腔科,云南昆明650111 [2]昆明医科大学,云南昆明650500

出　　处：《昆明医科大学学报》2017年第7期16-21,共6页Journal of Kunming Medical University

基　　金：国家自然科学基金资助项目(81360163)

摘　　要：目的观察Caspase-9在大鼠髁突软骨与长骨生长板软骨发育中的表达变化,探讨Caspase-9在软骨细胞发育中的意义.方法建立SD大鼠不同发育阶段(E15d^P30d)髁突软骨与长骨生长板软骨发育的动物模型,采用免疫组化检测Casepase-9在2种软骨中的表达变化.结果大鼠髁突软骨Caspase-9阳性表达强于生长板软骨(P<0.05),且生长板软骨阳性细胞的分布规律与髁突基本一致.胎鼠Caspase-9阳性主要表达于增殖层;出生后主要表达于成软骨层及肥大层.结论 Caspase-9参与了髁突软骨及生长板软骨的生长发育,由其所介导的细胞凋亡可能在软骨发育中发挥重要作用.且Caspase-9对于髁突软骨和生长板软骨的发育可能存在某些相同或相似的分子机制.Objective To investigate the different expression of Caspase-9in condylar cartilage and growth plate cartilage and to explore the role of Caspase-9in the development of cartilage.Methods Animal models of condylar cartilage and growth plate cartilage of SD rat were established during different development stages(E15d-P30d).The expressions of Caspase-9were examined by immuno-histochemical staining.Results Positive xpression of Caspase-9expression in condylar cartilage was stronger than that in growth plate cartilage(P<0.05).The distribution of positive cells in growth plate cartilage is consistent with those in the condylar cartilage.Caspase-9positive of fetal rat mainly expressed in the proliferative zone and expressed in the chondroblast zone and hypertrophic zone after birth.Conclusion Caspase-9is involved in the growth and development of condylar cartilage and growth plate cartilage,which may play an important role in the development of cartilage.Caspase-9may be of the same or similar molecular mechanismfor the development of condylar cartilage and growth plate cartilage.

关 键 词：髁状突软骨 生长板软骨 发育 CASPASE-9 

分 类 号：R329.28[医药卫生—人体解剖和组织胚胎学]