趋化因子受体CX3CR1基因T280M多态性在云南彝族人群中的分布  

The T280M Polymorphisms of Fractalkine Receptor CX3CR1 in a Yi Nationality Population of Yunnan Province

作　　者：陈志彬 莫艳萍[2] 杨钰 陈灿昆 杨靖宜 万瑞雪 范志祥[4] 龙莉[4] CHEN Zhi-bin;MO Yan-ping;YANG Yu;CHEN Can-kun;YANG Jing-yi;WAN Rui-xue;FAN Zhi-xiang;LONG Li(Kunming Medical University,Kunming Yunnan 650500;Dept.of Geriatrics,The 1st Affiliated Hospital of Kunming Medical University,Kunming Yunnan 650032;Reproductive Center,The 2nd Affiliated Hospital of Kunming Medical University,Kunming Yunnan 650101;Dept.of Cell Biology and Genetics,Kunming Medical University,Kunming Yunnan 650500,China)

机构地区：[1]昆明医科大学,云南昆明650500 [2]昆明医科大学第一附属医院老干科,云南昆明650032 [3]昆明医科大学第二附属院生殖医学中心,云南昆明650101 [4]昆明医科大学细胞生物学和医学遗传学教研室,云南昆明650500

出　　处：《昆明医科大学学报》2017年第8期35-39,共5页Journal of Kunming Medical University

基　　金：国家自然科学基金资助项目(81160164);云南省自然科学基金资助项目(2008CD117);昆明医科大学大学生创新性实验计划基金资助项目(昆医大[2017]2号)

摘　　要：目的研究云南省晋宁县彝族人群CX3CR1基因T280M多态性分布的情况,为探讨CX3CR1基因相关性疾病的发病机制奠定基础.方法采用PCR-RFLP技术对居住在云南省晋宁县268例彝族健康个体的CX3CR1基因T280M多态性进行分析,计算MM、MT、TT基因型频率和M、T等位基因频率,与其他人群该位点多态性进行比较.结果云南省晋宁县彝族CX3CR1基因的MM、MT、TT基因型频率分别为1.12%、11.94%和86.94%,M和T等位基因频率分别为7.09%和92.91%.与汉族、维吾尔族、希腊、法国、塞尔维亚、印度、意大利和高加索人群比较,无论是基因型分布还是基因频率差异有统计学意义(P<0.05);但与景颇族比较中差异无统计学意义(P>0.05).结论 CX3CR1基因T280M多态性分布存在民族、种族差异,且种族间的差异大于民族间的差异.Objective To investigate the distribution of the CX3CR1gene T280M polymorphism in a Yi nationality population of Yunnan province,and lay the foundation of exploring the pathogenesis of CX3CR1gene ralated diseases.Methods We studied268healthy individuals of Yi nationality in a rural area of Jinning,Yunnan province.Genotype determination of the CX3CR1gene T280M polymorphism was performed using Polymerase Chain Reaction-Restriction fragment Length Polymorphism(PCR-RFLP)technique.The frequencies of MM,MT,TT genotypes and M,T alleles were calculated.We conducted some analysis by comparing with results of other populations collected from previous studies.Results In the healthy Yi nationality population of Yunnan province,CX3CR1genotype frequencies for the MM,MT and TT genotypes were1.12%,11.94%and86.94%.Deriving from those,the allele frequencies for the M and T allele were7.09%and92.91%respectively.Significant differences in the distribution of CX3CR1genotypes and the allele frequencies were observed between the Yunnan Yi people and other nationality populations(The han,The uighurs,Greece,France,Serbia,India,Italy and The Caucasus)(P<0.05);whereas there was no significant difference with the Jingpo nationality(P>0.05).Conclusions Differences of the genotype or allele frequency of the CX3CR1T280M polymorphism exist in different nationalities and different races,and the national difference is more obvious than ethnic ones.

关 键 词：CX3CR1基因 基因多态性 彝族 分布 

分 类 号：R181[医药卫生—流行病学]