绿脓假单胞菌感染中耳上皮细胞可以激活TGF-β1 Smad信号通路  

Infection of Pseudomonas aeruginosa Activates TGF-β1 Smad Signaling Pathway in Human Middle Ear Epithelial Cells

作　　者：龙瑞清 刘卓慧[1] 张帆[1] 李书聆[1] 余咏梅[1] 阮标[1] LONG Rui-qing;LIU Zhuo-hui;ZHANG Fan;LI Shu-ling;YU Yong-mei;RUAN Biao(Dept. of ENT,First Affiliated Hospital of Kunming Medical University,Kunming Yunnan 650031,China)

机构地区：[1]昆明医科大学第一附属医院耳鼻咽喉科,云南昆明650031

出　　处：《昆明医科大学学报》2017年第10期65-69,共5页Journal of Kunming Medical University

基　　金：国家自然科学基金资助项目(81660175);云南省卫生科技计划基金资助项目(2014NS193)

摘　　要：目的探讨绿脓假单胞菌(pseudomonas aeruginosa,P.aeruginosa)在感染中耳上皮细胞过程中的作用机制.方法采用Real-time PCR和Western blotting方法检测绿脓假单胞菌感染中耳上皮细胞后,转化生长因子-β1(TGF-β1)和Smad2蛋白的mR和蛋白水平以及Smad7蛋白的磷酸化水平.结果绿脓假单胞菌在感染中耳上皮细胞过程中显著上调TGF-β1和Smad2的m RNA和蛋白水平,同时抑制了Smad7的磷酸化;突变型(Opr F)绿脓假单胞菌和采用蛋白激酶C(protein kinase C,PKC)抑制剂Bisindolylmaleimide I处理中耳上皮细胞均可以下调TGF-β1和Smad2的m RNA和蛋白水平,同时促进Smad7蛋白的磷酸化.结论绿脓假单胞菌感染中耳上皮细胞可以激活TGF-β1/Smad信号通路.Objective To study the molecular mechanism of the infection process of Pseudomonas aeruginosa in human middle ear epithelial cells(HMEECs).Methods Real-time PCR and Western blotting method were used to detect the mRNA and protein levels of TGF-β1and Smad2,and the phosphorylation of Smad7in the infection process of Pseudomonas aeruginosaonhuman middle ear epithelial cells.Results Infection of Pseudomonas aeruginosa in human middle ear epithelial cells significantly increased the mRNA and protein levels of TGF-β1and Smad2,and also inhibited the phosphorylation of Smad7.Mutation of OprF and PKC inhibitor Bisindolylmaleimide I could derease the mRNA and protein levels of TGF-β1and Smad2and the enhanced the phosphorylation of Smad7compared with Pseudomonasaeruginosa infected HMEECs.Conclusion Infection of Pseudomonas aeruginosaonhuman activates the TGF-β1Smad signaling pathway in middle ear epithelial cells.

关 键 词：绿脓假单胞菌 人中耳上皮细胞 TGF-Β1/SMAD信号通路 蛋白激酶C 

分 类 号：R764.12[医药卫生—耳鼻咽喉科]