连花清瘟胶囊超高效液相指纹图谱研究  

Study on UPLC Fingerprints of Lianhua Qingwen Capsules

作　　者：陈育鹏 赵倩 王贵金[3] 张水英 毕丹 CHEN Yu-peng;ZHAO Qian;WANG Gui-jin;ZHANG Shui-ying;BI Dan(Hebei Province Institute of Integrated Traditional and Western Medicine, Shijiazhuang 050035, China;Beijing Yiling Pharmaceutical Co., Ltd, Beijing 102600, China;Shijiazhuang Yiling Pharmaceutical Co., Ltd, Shijiazhuang 050035, China)

机构地区：[1]河北省中西医结合医药研究院,河北石家庄050035 [2]北京以岭药业有限公司,北京102600 [3]石家庄以岭药业股份有限公司,河北石家庄050035

出　　处：《中国中医药信息杂志》2017年第12期77-80,共4页Chinese Journal of Information on Traditional Chinese Medicine

基　　金：国家自然科学基金青年基金(81503231);国家中药标准化项目(ZYBZH-C-HEB-12);北京市科技计划"十病十药"中药专项(Z161100001816022)

摘　　要：目的研究并建立连花清瘟胶囊指纹图谱。方法采用超高效液相色谱法(UPLC),色谱柱为Waters ACQUITY UPLC HSS T3(1.8μm,2.1 mm×100 mm),以甲醇-乙腈-0.1%磷酸溶液为流动相进行梯度洗脱,检测波长为239 nm,流速为0.3 m L/min,柱温为30℃。结果建立连花清瘟胶囊指纹图谱,共标定了32个共有峰,指认了其中9个共有峰,分别为新绿原酸(4号峰,来源于金银花和鱼腥草)、绿原酸(6号峰,来源于连翘、金银花和鱼腥草)、隐绿原酸(8号峰,来源于金银花和鱼腥草)、异连翘酯苷A(15号峰,来源于连翘)、连翘酯苷A(20号峰,来源于连翘)、槲皮苷(23号峰,来源于鱼腥草)、异绿原酸C(24号峰,来源于金银花)、连翘苷(26号峰,来源于连翘)、甘草酸(31号峰,来源于甘草),10批连花清瘟胶囊指纹图谱的相似度均大于0.96。结论本研究建立的方法具有良好的精密度、重复性和稳定性,为控制连花清瘟胶囊的质量提供了新方法。Objective To study and establish the UPLC fingerprints of Lianhua Qingwen Capsules.Methods The samples were separated with a Waters ACQUITY UPLC HSS T3column(100mm×2.1mm,1.8μm)by linear gradient elution.The wavelength for detection was set at239nm;mobile phase was set at a flow rate of0.3mL/min;the column temperature was set at30℃.Results UPLC fingerprints of Lianhua Qingwen Capsules were established with32common peaks.9of32common peaks were identified,including neochlorogenic acid(peak No.4,source from Lonicerae Japonicae Flos and Houttuyniae Herba),chlorogenic acid(peak No.6,source from Forsythiae Fructus,Lonicerae Japonicae Flos and Houttuyniae Herba),cryptochlorogenic acid(peak No.8,source from Lonicerae Japonicae Flos and Houttuyniae Herba),isoforsythiaside A(peak No.15,source from Forsythiae Fructus),forsythoside A(peak No.20,source from Forsythiae Fructus),quercitrin(peak No.23,source from Houttuyniae Herba),isochlorogenic acid C(peak No.24,source from Lonicerae Japonicae Flos),phillyrin(peak No.26,source from Forsythiae Fructus),glycyrrhizic acid(peak No.31,source from Glycyrrhizae Radix et Rhizoma).The similarities in10batches of Lianhua Qingwen Capsules samples were all above0.96.Conclusion The method is with good precision,repeatability and stability,which can be used as a new means for the quality control of Lianhua Qingwen Capsules.

关 键 词：连花清瘟胶囊 超高效液相色谱法 指纹图谱 

分 类 号：R284.1[医药卫生—中药学]