“动物组织中DNA的分离提取及含量测定”实验教学改革  

Experimental teaching reform on “Isolation,Extraction and Content Determination of DNA in Animal Tissues”

作　　者：赵玉红[1] 李欣[1] 李小菊[1] 周浩[1] 李登文[1] 石建党[1] 张金红[1] 赵立青[1] Zhao Yuhong;Li Xin;Li Xiaoju;Zhou Hao;Li Dengwen;Shi Jiandang;Zhang Jinhong;Zhao Liqing(Biological Experimental Teaching Center, Nankai University, Tianjin 300071, China)

机构地区：[1]南开大学生物实验教学中心,天津300071

出　　处：《实验技术与管理》2017年第11期214-216,220,共4页Experimental Technology and Management

基　　金：国家基础学科人才培养基金"条件建设项目"(J1310003);南开大学2014年教学改革项目

摘　　要：对经典生物化学实验项目"动物组织中DNA的分离提取及含量测定"实验进行改革。在传统浓盐法提取基因组DNA、二苯胺法检测DNA含量的实验基础上,新增试剂盒方法提取DNA以及超微量分光光度计检测DNA两大现代实验技术内容,对经典实验进行拓展、升级。此外,针对实验中比色杯污染影响检测结果精密度和准确性的困扰难题,提出2种简便易行的清洗方法,提高实验数据的真实性和有效性。The reform is carried out on the classical biochemical experiment,i.e.,Isolation,Extraction and Content Determination of DNA in Animal Tissues.On the basis of the traditional concentrated salt method of extracting genomic DNA and the diphenylamine method of detecting the DNA content,the two major modern experimental technical contents are newly added,i.e.,the reagent kit method is used to extract DNA and the ultra micro spectrophotometer is used to detect DNA,which has expanded and upgraded the classical experiment.In addition,in view of the problem in the experiment that the colorimetric cup contamination affects the precision and accuracy of the detection results,two simple and convenient cleaning methods are put forward to improve the authenticity and validity of the experimental data.

关 键 词：实验教学改革 DNA提取 DNA检测 

分 类 号：Q5-33[生物学—生物化学] G642.423[文化科学—高等教育学]