极端低气压处理后低温高温交替对发状念珠藻的光合生理活性的影响  

Effects of Low-high Temperature Alternations on Photosynthetic Physiology of Nostoc flagelliforme after Extreme Low Gas Pressure Exposure

作　　者：刘颖慧[1] 褚亚东[1] 陆洪斌[1] 曹旭鹏[1] 薛松[1] LIU Yinghui;CHU Yadong;LU Hongbin;CA0 Xupeng;XUE Song(Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China)

机构地区：[1]中国科学院大连化学物理研究所,大连116023

出　　处：《载人航天》2017年第6期829-834,共6页Manned Spaceflight

摘　　要：为考察发状念珠藻在类地行星环境中生存的可行性,搭建了模拟类地行星环境的极端低气压实验装置,并研究极端低气压与低温-高温交替作用对发状念珠藻光合生理活性的影响。结果显示,在气体组成为CO_2和N_2(体积比为95∶5)的氛围中,在-20℃、100 Pa、600 Pa和1200 Pa气压下,分别处理发菜4天、7天、15天、30天和60天,发菜的呼吸和光合放氧速率与未处理样品没有差别。此外,不同气压下,-20℃到25℃交替处理发菜10天、20天和30天,发菜的光合及呼吸速率与正常相比也没有差异。以上结果表明,发菜能够生存在极端低气压下的低温-高温交替环境。通过分析样品的脂肪酸组成和游离脯氨酸含量发现,处理后样品的游离脯氨酸含量升高,脂肪酸组成也发生了相应的变化,这些变化是维持发菜耐受极端低气压和低温-高温交替的物质基础。In order to examine whether Nostoc flagelliforme can survive on terrestrial planets or not,an experimental apparatus providing extremely low air pressure lor simulating terrestrial planet envi-ronment was constructed,and theeffects of low-high temperature alternation on the photosynthetic physiology ol Nostoc flagelliforme under extremely low air pressures were investigated.It was found thatunder a gas atmosphereof CO2/N2(95/5,v/v),the respiration and photosynthetic oxygen re-lease rate of N.flagelliforme were not affected when it was treated with various extremely low gas pressures(100Pa,600Pa,1200Pa)for4days,7days,15days,30days and60days at-20℃,respectively.In addition,when N.flagelliforme was subjected to-20℃to-25℃alternation un-der different low gas pressuresfor10days,20days and30days,the respiration and photosynthetic oxygen release rates showed also no difference as compared with the control.These results indicated that N.flagelliforme might survive under an extremely low gas pressure with low-high temperature al-ternations.Furthermore,the free proline contents increased and fatty acid composition changed when N.flagelliforme was treated with the extremely low gas pressure and low-high temperature al-ternations,which could be the material foundations for the tolerance capability of N.flagelliforme in response to these extreme environments.

关 键 词：发菜 低气压 光合 呼吸 脯氨酸 

分 类 号：Q935[生物学—微生物学]