陈皮、藿香不同提取物对胃肠动力障碍大鼠胃肠平滑肌收缩活动及胃肠激素的影响  

Effects of extracts of pericarpium citri reticulatae and pogostemon cablin on the contraction of gastrointestinal smooth muscle and gastrointestinal hormones in rats with gastrointestinal motility disorder

作　　者：何占坤 张国梁[1] 唐方 杜进超 靳文杰[1] HE Zhan-kun;ZHANG Guo-liang;TANG Fang;DU Jin-chao;JIN Wen-jie(Department of Gastroenterology, Tianjin First Center Hospital, Tianjin 300192, China;Department of Traditional Chinese Medicine, Tianjin Medical University General Hospital;Department of Endocrinology, Tianjin Ninghe District Hospital of Traditional Chinese Medicine)

机构地区：[1]天津市第一中心医院消化科,300192 [2]天津医科大学总医院 [3]天津市宁河区中医医院

出　　处：《天津医药》2017年第11期1175-1179,共5页Tianjin Medical Journal

摘　　要：目的研究陈皮、藿香不同提取物对胃肠动力障碍大鼠胃肠平滑肌收缩活动及相关胃肠激素的影响。方法70只Wistar大鼠随机分为正常对照组、模型组、陈皮水提液组、橙皮苷组、藿香水提液组、藿香水提液+挥发油组、百秋李醇组,每组10只。除正常对照组不予处理外,其余各组均采用肢体缺血再灌注(LIR)方法建立胃肠动力障碍模型,建模后分别予以相应药物处理。观察不同提取物对大鼠胃体及小肠平滑肌条收缩振幅的影响,放射免疫法检测血浆胃动素(MOT)、血清胃泌素(GAS)及胃窦组织胆囊收缩素(CCK)、生长抑素(SS)水平的变化。结果模型组大鼠胃体、小肠平滑肌条收缩振幅较正常对照组均明显下降(均P<0.05),同时伴随着血清GAS、血浆MOT水平的降低和胃窦组织CCK、SS水平的升高(均P<0.05)。陈皮水提液、橙皮苷、藿香水提液、藿香水提液+挥发油均可显著提高LIR大鼠胃体、小肠平滑肌条收缩振幅及血清GAS水平,降低胃窦组织CCK、SS水平(均P<0.05);而对血浆MOT水平无影响(P>0.05)。与模型组相比,百秋李醇对胃体、小肠平滑肌条收缩振幅及MOT、GAS、CCK、SS水平均无影响(P>0.05)。在调节胃肠平滑肌收缩活动及GAS、CCK、SS水平方面,陈皮水提液的药效优于橙皮苷(均P<0.05),藿香水提液加挥发油后优于单纯藿香水提液(均P<0.05)。结论陈皮、藿香有效提取物可不同程度调节胃肠动力障碍大鼠胃肠平滑肌收缩活动及血清GAS、胃窦组织CCK、SS水平。Objective To investigate the effects of different extracts of pericarpium citri reticulatae(PCR)and pogostemon cablin benth(PCB)on the contraction of gastrointestinal smooth muscle and the level of gastrointestinal hormones in rat model of gastrointestinal motility disorder.Methods Seventy Wistar rats were randomly divided into groups of control,model,water extractive of PCR,hesperidin,water extractive of PCB,water extractive of PCB plus volatile oil and patchouli alcohol,ten rats in each group.Except the control group,the rest groups were established gastrointestinal motor disorder model via limb ischemia-reperfusion(LIR).After modeling rats of groups were intervened with corresponding extracts.The effects of different extracts on contraction amplitude of corpora ventriculi and small intestine smooth muscle were observed.The levels of gastrointestinal hormones including motilin(MOT),gastrin(GAS),cholecystokinin(CCK)and somatostatin(SS)were detected by radioimmunoassay.Results The contraction amplitudes of corpora ventriculi and small intestine smooth muscle were decreased(P<0.05),the serum level of GAS and plasma level of MOT were also significantly decreased,while CCK and SS levels in the gastric antrum were significantly increased in model group than those of the control group(P<0.05).Water extractive of PCR,hesperidin,water extractive of PCB and water extractive of PCB+patchouli oil can increase the contraction amplitudes of corpora ventriculi and small intestine smooth muscle,increase the serum level of GAS and reduce levels of CCK and SS in the gastric antrum(P<0.05),whereas showed no influence in the plasma level of MOT(P>0.05)].Compared with model group,patchouli alcohol showed no influence in the contraction of gastrointestinal smooth muscle and levels of MOT,GAS,CCK and SS(P>0.05).In the aspect of regulating the contraction of gastrointestinal smooth muscle and the level of GAS,CCK and SS,the pharmacological effect of PCR water extract was better than that of hesperidin(P<0.05),while water extractive of

关 键 词：陈皮 广藿香 大鼠 Wistar 再灌注损伤 促胃动素 胃泌素类 胆囊收缩素 生长抑素 胃肠动力障碍 

分 类 号：R285.5[医药卫生—中药学] R347.92[医药卫生—中医学]