Systematic Analysis of Gene Expression in Western Flower Thrip( Frankliniella occidentalis Pergande ) before and after Infection with Impatiens Necrotic Spot Virus(INSV)  

作　　者：Jiani LIU Shengguang XU Yuyu ZHANG Zebin CHEN Rong LU Lei YU 

机构地区：[1]Yunnan Urban Agricultural Engineering and Technologcal Research Center/College of Agriculture,Kunming University,Kunming 650214,China [2]Yunnan Bio-carbon Ennineering Technology Research Center,Kunming University,Kunming 650214,China

出　　处：《Agricultural Biotechnology》2017年第5期28-31,46,共5页农业生物技术（英文版）

基　　金：Supported by National Natural Science Foundation of China(31301665)

摘　　要：Impatiens necrotic spot virus( INSV) is an important plant virus identified in recent years. Western flower thrip( Frankliniella occidentalis) has been confirmed to be the most efficient vector for INSV. In the present study,Illumina's second-generation sequencing technique was used to determine the variation in gene expression at the transcriptome level in western flower thrip before and after infection with INSV. By aligning 168 188 unigenes of western flower thrip to the NCBI protein database,47 558 unigenes were annotated,accounting for 28. 27% of the total. Species classification revealed that 58. 7% of the annotated unigenes of western flower thrip did not match any other species,and 15. 4% of had a match in Zootermopsis navadensis,12. 4% in Mus musculus,7. 3% in Mustela putorius,3. 4% in Theileria parva,and 2. 7% in Tribolium castaneum. By analyzing DEGs between INSV-free and INSV-carrying western flower thrips,we found that 11 genes were upregulated,and 9 genes were downregulated in western flower thrip after INSV infection. In addition,cell cycle and ubiquitin-proteasome pathway in western flower thrip were significantly infected by INSV infection.Impatiens necrotic spot virus( INSV) is an important plant virus identified in recent years. Western flower thrip( Frankliniella occidentalis) has been confirmed to be the most efficient vector for INSV. In the present study,Illumina's second-generation sequencing technique was used to determine the variation in gene expression at the transcriptome level in western flower thrip before and after infection with INSV. By aligning 168 188 unigenes of western flower thrip to the NCBI protein database,47 558 unigenes were annotated,accounting for 28. 27% of the total. Species classification revealed that 58. 7% of the annotated unigenes of western flower thrip did not match any other species,and 15. 4% of had a match in Zootermopsis navadensis,12. 4% in Mus musculus,7. 3% in Mustela putorius,3. 4% in Theileria parva,and 2. 7% in Tribolium castaneum. By analyzing DEGs between INSV-free and INSV-carrying western flower thrips,we found that 11 genes were upregulated,and 9 genes were downregulated in western flower thrip after INSV infection. In addition,cell cycle and ubiquitin-proteasome pathway in western flower thrip were significantly infected by INSV infection.

关 键 词：WESTERN FLOWER thrip (Frankliniella occidentalis) IMPATIENS NECROTIC spot virus (INSV) Transcriptome Gene expression 

分 类 号：S43[农业科学—农业昆虫与害虫防治]