Identification of Pathogens from Rotted Potato Tubers in Ventilated Storage House of Huade County,Inner Mongolia during the Storage Period  

作　　者：Kai ZHANG Xizhuo WANG Jie SUN Qin Yang Haiting SUN 

机构地区：[1]Chinese Academy of Agricultural Engineering/Key Laboratory of Agroproducts Postharvest Handling,Ministry of Agriculture,Beijing 100125,China

出　　处：《Agricultural Biotechnology》2017年第5期61-65,共5页农业生物技术（英文版）

基　　金：Supported by National Key Research and Development Program of China"Research and Demonstration of Derogation Technical Equipment and Model for Postpartum Storage and Transportation of Potato Tubers"(2016YFD0401301)

摘　　要：Rotted potato samples were collected from ventilated storage house of Huade County,Inner Mongolia from October 2015 to April 2016. Pathogens were isolated,purified and cultured with tissue separation method,and identified by morphological observation and molecular identification. The results showed that there were five main diseases that caused potato rotting in Huande County,Inner Mongolia during the storage period,including dry rot,late blight,early blight,ring rot and soft rot. Specifically,potato dry rot was the most serious disease,which was induced by four pathogens: Fusarium sambucinum,Fusarium Link,Fusarium acuminatum and Fusarium equiseti. Late blight caused by Phytophthora infestans and early blight caused by Alternaria solani could easily result in complex infection,which were mostly found in seriously rotted potato tubers. Moreover,these potatoes were always co-infected with fungal diseases at late stage of storage or even subject to complex infection with bacteriosis in serious cases. This study provided theoretical basis for the prevention of potato diseases during the storage period.Rotted potato samples were collected from ventilated storage house of Huade County,Inner Mongolia from October 2015 to April 2016. Pathogens were isolated,purified and cultured with tissue separation method,and identified by morphological observation and molecular identification. The results showed that there were five main diseases that caused potato rotting in Huande County,Inner Mongolia during the storage period,including dry rot,late blight,early blight,ring rot and soft rot. Specifically,potato dry rot was the most serious disease,which was induced by four pathogens: Fusarium sambucinum,Fusarium Link,Fusarium acuminatum and Fusarium equiseti. Late blight caused by Phytophthora infestans and early blight caused by Alternaria solani could easily result in complex infection,which were mostly found in seriously rotted potato tubers. Moreover,these potatoes were always co-infected with fungal diseases at late stage of storage or even subject to complex infection with bacteriosis in serious cases. This study provided theoretical basis for the prevention of potato diseases during the storage period.

关 键 词：POTATO STORAGE diseases PATHOGEN IDENTIFICATION CTAB method PCR amplification Molecular IDENTIFICATION 

分 类 号：S435.32[农业科学—农业昆虫与害虫防治]