烟草种质资源TI203对烟草花叶病的抗性特点  

Resistance characteristics of tobacco germplasm resource TI203 to Tobacco mosaic virus

作　　者：于海芹[1] 饶思莲 赵丹阳 刘勇[1] 李永平[1] YU Hai-qin;RAO Si-lian;ZHAO Dan-yang;LIU Yong;LI Yong-ping(Yunnan Academy of Tobacco Agriculture Science/Key Laboratory of Tobacco Biotechnological Breeding/National Tobacco Genetic Engineering Research Center,Kunming 650021,China;Zhaotong Branch,Yunnan Tobacco Company,Zhaotong,Yunnan 657000,China;Institute of Biotechnology,College of Agriculture and Biotechnology,Zhejiang University/Key Laboratory of Crop Diseases and Pests Molecular Biology,Ministry of Agriculture,Hangzhou 310058,China)

机构地区：[1]云南省烟草农业科学研究院/烟草行业烟草生物技术育种重点实验室/国家烟草基因工程研究中心,昆明650021 [2]云南省烟草公司昭通市公司,云南昭通657000 [3]浙江大学农业与生物技术学院生物技术研究所/农业部作物病虫分子生物学重点实验室,杭州310058

出　　处：《南方农业学报》2017年第11期2004-2009,共6页Journal of Southern Agriculture

基　　金：中国烟草总公司科技项目(110201401001);中国烟草总公司云南省公司科技项目(2014YN02)

摘　　要：【目的】系统了解烟草种质资源TI203抗烟草花叶病毒(Tobacco mosaic virus,TMV)的特点,为烟草抗病育种的亲本选择提供更丰富信息。【方法】以烟草种质资源TI203为材料,采用TMV病毒接种、病情调查、病毒外壳蛋白累计量检测、TMV复制水平测定及日灼现象观察等方法,研究TI203对TMV的抗性特异性、TI203接种TMV普通株系(TMV-U1株系)后的症状、病毒复制水平等。【结果】TI203只对TMV表现无症状的抗病特点,接种马铃薯Y病毒(PVY)和黄瓜花叶病毒(CMV)则表现出典型症状。接种TMV-U1株系后14 d,Western blotting检测结果显示,TI203叶片病毒复制水平明显低于对照品种;用表达GFP的TMV侵染性克隆浸润接种发现,TI203对TMV的抗性发生在病毒复制水平。在高温和强日照条件下,TI203生长迅速的中上部叶片会发生日灼现象,且难以恢复。【结论】TI203只对TMV表现无症状,其对TMV的抗性发生在病毒复制水平。【Objective】This research was conducted to understand the resistance characteristics of tobacco germplasm resource TI203to Tobacco mosaic virus(TMV),in order to provide more information for parent selection in disease resistant breeding of tobacco.【Method】Tobacco germplasm resource TI203was taken as experimental material.The resistance specificity of TI203to TMV,and symptom and level of virus replication of TI203after inoculated with TMV ordinary strain(TMV-U1strain)were studied by TMV inoculation,disease investigation,virus coat protein accumulation detection,TMV replication level determination and observation on sunburn phenomenon.【Result】The results showed that TI203exhibited a symptomless reaction to TMV,but typical symptoms to Potato virus Y(PVY)and Cucumber mosic virus(CMV)after inoculation.Western blotting detection results indicated that the level of virus replication in TI203leaves was obviously lower than that of control variety14d after inoculated with TMV-U1strain.Resistance of TI203to TMV was observed at virus replication level by using TMV-GFP infectious clone infiltration inoculation.Under high temperature and strong sunlight conditions,sunburn phenomena always occurred on fast-growing middle and upper leaves of TI203,and the leaves were difficult to recover.【Conclusion】TI203is symptomless to TMV and its resistance to TMV occurs at virus replication level.

关 键 词：烟草种质 TI203 烟草花叶病毒(TMV) 抗病性 

分 类 号：S286.3[农业科学—农业水土工程]