细胞无血清培养现状概述  

Overview of Current Status of Serum-Free Cell Culture

作　　者：郭芳睿 秦俊红 李彦霖 康玮笠 王晨曦 朱玲[1] 徐志文[1] GUO Fang-Rui;QIN Jun-Hong;LI Yan-Lin;KANG Wei-Li;WANG Chen-Xi;ZHU Ling;XU Zhi-Wen(College of Veterinary Medicine, Sichuan Key Laboratory of Animal Disease and Human Health, Sichuan Agricultural University, Chengdu 611130, China)

机构地区：[1]四川农业大学动物医学院动物疫病与人类健康四川省重点实验室,四川成都611130

出　　处：《生物技术通讯》2017年第6期865-870,共6页Letters in Biotechnology

基　　金：四川省科技支撑计划(2017NZ0038);"十二五"农村领域国家科技计划(2015BAD12B04-2.3)

摘　　要：在动物细胞培养过程中,血清的存在能满足细胞的生长繁殖需求,但实际生产中血清提取工艺复杂,成本高昂,给大规模生产带来困难,不利于商业化开发。常见细胞源疫苗血清残留对疫苗免疫效果有很大的影响,而无血清培养基避免了血清所带来的血源性污染等问题,其成分和含量明确,制备流程简易,提高了实验的稳定性和准确性,有利于获得高质量的生物产品,与天然培养基等传统的培养基相比有着许多优势。随着生物科学的发展,细胞无血清培养技术的创新和应用已成为生物工程重点研究课题。我们简要综述了无血清驯化细胞的发展历程、应用,及无血清培养基的组成成分、优缺点等内容,以期对未来细胞培养技术的发展进程提供助力。In the process of culturing animal cells,the existence of serum can meet the needs of cellular growth and breeding,but in practical production,it is complicated to extract serum and it's expensive,also it brings difficulties for mass production and it goes against commercial development.The residual from the serum of common cell-derived vaccine has a great influence on the immune effect of vaccine,however,serum-free culture medium avoids some problems brought by serum such as hematogenous contamination,the component and content of it are definite,the process to prepare is simple,also,the serum-free culture medium can improve the stability and accuracy,and it is beneficial to get biological products of high quality.So it has advantages over traditional culture medium such as natural culture medium.With the development of bioscience,the innovation and application of serum-free culture technology have been a key research subject in bioscience.The summary included the development and application of domesticating cells without serum,the component of serum-free culture medium,its advantages and disadvantages and so on,looking forward to providing some help for the development of cellculture technology in the future.

关 键 词：无血清 培养 细胞株 

分 类 号：Q813.1[生物学—生物工程]