Effect of ultraviolet mutagenesis on heterotrophic strain mutation and bioleaching of low grade copper ore  

作　　者：WU Ai-xiang HU Kai-jian WANG Hong-jiang ZHANG Ai-qing YANG Ying 吴爱祥;胡凯建;王洪江;张爱卿;杨莹

机构地区：[1]School of Civil and Environmental Engineering,University of Science and Technology Beijing [2]School of Resources and Environmental Engineering,Jiangxi University of Science and Technology

出　　处：《Journal of Central South University》2017年第10期2245-2252,共8页中南大学学报（英文版）

基　　金：Project(2012BAB08B02)supported by the National Key Technologies R&D Program for the 12th Five-year Plan,China;Projects(51304011,51374035)supported by the National Natural Science Foundation of China

摘　　要：The effect of ultraviolet mutagenesis on a heterotrophic strain(Providencia JAT-1) mutation was studied and bioleaching of low grade copper ore with mutant bacteria was investigated. The results show that the activity of bacteria was improved after ultraviolet mutagenesis; the best irradiation time was 120 s. Compared to the original bacteria, the cells density of mutant bacteria at stationary phase increased by 26% and ammonia produced by mutant bacteria increased by 12%. Higher activity of bacteria leads to a higher copper extraction rate. The bioleaching performance of Providencia JAT-1 was improved after UV mutagenesis. The copper extraction rate with mutant bacteria increased by 10.6% compared to the original bacteria. The ore surface was corroded and the fine particles were absent after bioleaching. Free copper oxide and copper silicates could be leached out easily by using JAT-1; a small part of the copper sulfide can also be leached out. Bioleaching using JAT-1 is more effective than ammonia leaching and copper extraction rate with mutant bacteria was 21.1% higher than that by ammonia leaching under the same condition.The effect of ultraviolet mutagenesis on a heterotrophic strain(Providencia JAT-1) mutation was studied and bioleaching of low grade copper ore with mutant bacteria was investigated. The results show that the activity of bacteria was improved after ultraviolet mutagenesis; the best irradiation time was 120 s. Compared to the original bacteria, the cells density of mutant bacteria at stationary phase increased by 26% and ammonia produced by mutant bacteria increased by 12%. Higher activity of bacteria leads to a higher copper extraction rate. The bioleaching performance of Providencia JAT-1 was improved after UV mutagenesis. The copper extraction rate with mutant bacteria increased by 10.6% compared to the original bacteria. The ore surface was corroded and the fine particles were absent after bioleaching. Free copper oxide and copper silicates could be leached out easily by using JAT-1; a small part of the copper sulfide can also be leached out. Bioleaching using JAT-1 is more effective than ammonia leaching and copper extraction rate with mutant bacteria was 21.1% higher than that by ammonia leaching under the same condition.

关 键 词：ULTRAVIOLET MUTAGENESIS BIOLEACHING HETEROTROPHIC STRAIN ammonia low grade copper ORE 

分 类 号：Q939.97[生物学—微生物学] TD925[矿业工程—选矿] TD952