小剂量氢化可的松与血必净对急性肺损伤大鼠作用比较  

Comparison of the effects of low-dose hydrocortisone and Xuebijing injection on acute lung injury in rats

作　　者：郭琳瑛[1] 李红日 张琪[1] 崔小岱 宋国维[1] Guo Linying;Li Hongri;Zhang Qi;Cui Xiaodai;Song Guowei(Department of Critical Care Medicine,Children′s Hospital Affiliated to Capital Institute of Pediatrics,Beijing 100020, China;Department of Critical Care Medicine, Beijing Jingdu Children′s Hospital, Beijing 102208, China;3Department of Central Clinical Laboratory, Children′s Hospital Affiliated to Capital Institute of Pediatrics,Beijing 100020, China)

机构地区：[1]首都儿科研究所附属儿童医院重症医学科,北京100020 [2]北京京都儿童医院重症医学科,100020 [3]北京京都儿童医院中心试验室,100020

出　　处：《中华临床医师杂志（电子版）》2017年第11期1889-1893,共5页Chinese Journal of Clinicians(Electronic Edition)

摘　　要：目的观察小剂量氢化可的松与血必净注射液对大肠埃希菌导致的大鼠急性肺损伤(ALI)的保护作用。方法大肠埃希菌[O111B4,(4.4~5.6)×1012CFU/L]3 ml/kg气管注射制作大鼠肺内源性ALI模型,设氢化可的松干预组[HC组,腹腔注射氢化可的松5 mg/(kg·d),共2天]、血必净干预组[X组,腹腔注射血必净注射液5 ml/(kg·d),共2 d]、模型对照组[C组,腹腔注射生理盐水5 ml/(kg·d),共2天]、正常对照组[N组,气管注射3 ml/(kg·d)生理盐水,腹腔注射生理盐水5 ml/(kg·d),共2天]。各组分别于末次给药后12 h放血处死动物,留血清测TNF-α、IL-8水平。取左肺计算肺指数。结果 (1)HC组、X组、C组病死率无统计学意义(χ~2=0.194,P=1.000);(2)TNF-α水平:HC组及X组[分别为(80.31±11.87)ng/L,(89.42±22.76)ng/L]均明显低于C组[(125.68±27.91)ng/L](P<0.05),HC组与X组差别无统计学意义(P>0.05);(3)IL-8水平:HC组与X组[分别为(175.01±40.29)ng/L,(214.38±56.03)ng/L]均明显低于C组[(266.48±56.09)ng/L](P<0.05),HC组与X组差别无统计学意义(P>0.05);(4)肺指数:HC组与X组[分别为(0.318±0.058)%,(0.352±0.046)%]均明显低于C组[(0.412±0.055)%](P<0.05),HC组与X组差别无统计学意义(P>0.05)。结论小剂量氢化可的松及血必净治疗大肠杆菌导致的大鼠ALI均能明显降低血清促炎因子的水平,减轻肺水肿,两者作用相似。Objective To investigate the efficacy of low-dose hydrocortisone and Xuebijing injection on acute lung injury(ALI)induced by Escherichia coli(E.coli)in rats.Methods ALI model was induced in Wistar rats by intratracheal E.coli injection[3ml/kg,O111B4,(4.4~5.6)×1012CFU/L],twelve hours later the survived rats were randomly divided into hydrocortisone group(HC)treated with hydrocortisone for two days[5mg/(kg?d),intraperitoneally],Xuebijing group(X)treated with Xuebijing injection for two days[5ml/(kg?d),intraperitoneally],and ALI control group(C)treated with normal saline for two days(5ml/(kg?d),intraperitoneally).And there was a normal control group(N)that received intratracheal E.coli(3ml/kg)injection and was treated with intraperitoneal normal saline for two days[5ml/(kg?d)].Twelve hours after the treatment the rats were killed,and the levels of TNF-and IL-8in serum were measured by ELASA,then the lung index was calculated.Results There was no difference in mortality among group HC,X and C(χ2=0.194,P=1.000).The levels of TNF-in serum in group HC and X[(80.31±11.87)ng/L and(89.42±22.76)ng/L,respectively]were significantly lower than that in group C[(125.68±27.91)ng/L](P<0.05),but there was no significant difference between group HC and X(P>0.05);IL-8in group HC and X[(175.01±40.29)ng/L and(214.38±56.03)ng/L,respectively]were significantly lower than that in group C[(266.48±56.09)ng/L](P<0.05),but there was no significant difference between group HC and X(P>0.05);The lung index in group HC and X[(0.318±0.058)%and(0.352±0.046)%,respectively]were significantly lower than that in group C[(0.412±0.055)%](P<0.05),but there was no significant difference between group HC and X(P>0.05).Conclusion Both low-dose hydrocortisone and Xuebijing injection can inhibit the production of pro-inflammatory cytokines and attenuate pulmonary edema in rats with E.coli-induced ALI with similar effects.

关 键 词：急性肺损伤 氢化可的松 血必净注射液 细胞因子 大鼠 

分 类 号：R563.8[医药卫生—呼吸系统]