大鼠肾移植急性排斥反应调节性T细胞及其基因表达的机制研究  

Changes in regulatory T cells and their gene expression in acute rejection associated with renal transplantation in rats

作　　者：顾凤娟 隋晓露 张艾莎 许云鹏[1] 陈继红[1] Gu Fengjuan;Sui Xiaolu;Zhang Aisha;Xu Yunpeng;Chen Jihong(Department of Nephrology, the First Affiliated Hospital of Xinjiang Medical University, Urumqi 830054, China)

机构地区：[1]新疆医科大学第一附属医院肾内科,乌鲁木齐830054

出　　处：《中华临床医师杂志（电子版）》2017年第12期1930-1933,共4页Chinese Journal of Clinicians(Electronic Edition)

基　　金：国家自然科学联合基金项目-本地优秀青年人才培养项目(U1403321)

摘　　要：目的观察肾移植大鼠外周血中CD4+CD25+调节性T细胞及其表面标志物Foxp3、ABCC4、e IF6基因表达,结合白细胞介素-2(IL-2)和肿瘤坏死因子-γ(INF-γ)水平及肝、肾功能变化进行相关分析,探讨其在移植免疫排斥机制中的作用及意义。方法监测急排组与非急排组大鼠肝、肾功能;ELISA方法检测血浆中IL-2和INF-γ的表达水平;流式细胞仪检测CD4+、CD8+、CD4+CD25+Treg细胞亚群;荧光PCR检测CD4+CD25+Treg细胞Foxp3、ABCC4及e IF6 m RNA表达。结果与术前相比,急排组术后第7天血清肌酐、尿素氮均明显升高(P<0.05);急排组术后第7天IL-2及IFN-γ均升高,差异有统计学意义(P<0.05);急排组术后CD3+、CD4+、CD8+水平明显升高,差异有统计学意义(P<0.05);与非急排组相比,急排组术后CD4+CD25+Treg细胞表达降低,差异有统计学意义(P<0.05)。与非急排组相比,急排组术后Foxp3+基因表达量明显降低,ABCC4、e IF6基因表达量升高,差异有统计学意义(P<0.05)。结论 CD4+CD25+调节性T细胞在肾移植排斥反应中起到重要作用,CD4+CD25+Treg细胞Foxp3、ABCC4及e IF6 m RNA参与急性排斥反应的发生,检测其变化可辅助诊断急性排斥反应的发生。Objective To observe the expression of CD4+CD25+regulatory T cells in peripheral blood(Treg)and their gene expression(Foxp3,ABCC4,and eIF6)in acute rejection(AR)associated with renal transplantation in rats,in order to clarify their role in immune rejection.Methods Liver and kidney function was monitored in rats with AR and those with non-acute rejection(n-AR).IL-2and IFN-γlevels were detected by ELISA.CD4+,CD8+,and CD4+CD25+cells(Treg)in fresh blood were detected by flow cytometry.The expression of Foxp3,ABCC4,and eIF6was detected by fluorescent PCR.Results Compared with preoperative values,serum levels of creatinine and urea nitrogen,plasma levels of IFN-γand IL-2,and the levels of CD3+,CD4+,and CD8+cells were significantly increased in the AR group(P<0.05).The percentage of CD4+CD25+Treg cells was significantly lower,the expression of Foxp3was significantly decreased,and the expression of ABCC4and eIF6was significantly increased in the AR group compared with the n-AR group(P<0.05).Conclusion CD4+CD25+regulatory T cellsparticipate in immune rejection associated with renal transplantation,and detecting the expression of Foxp3,ABCC4,and eIF6in CD4+CD25+Treg cells can assist in the diagnosis of AR.

关 键 词：肾移植 急性排斥反应 CD4+CD25+调节性T细胞 Foxp3mRNA ABCC4mRNA eIF6mRNA 

分 类 号：R699.2[医药卫生—泌尿科学]