老年糖尿病肾病患者外周血NLRP3炎性体与肾损伤指标的相关性分析  

Correlation between NLRP3 Inflammasome and Renal Injury Indicators in the Elderly with Diabetic Nephropathy

作　　者：黄秀丽[1] 吴艳 王超平[2] 王昀[3] 王皓[4] Huang Xiuli;Wu Yan;Wang Chaoping;Wang Yun;Wang Hao(Department of Endocrinology,Suining Central Hospital, Suining City, Sichuan Province,629000,P.R.China;Department of Respiratory Medicine; Suining Central Hospital, Suining City, Sichuan Province,629000,P.R.China;Department of Gastroenterology,Xining,Qinghai Province,810000, P.R.China;ICU,Affiliated Hospital to Qinghai University,Xining,Qinghai Province,810000, P.R.China)

机构地区：[1]遂宁市中心医院内分泌科(原工作单位为青海大学附属医院内分泌科),四川遂宁629000 [2]遂宁市中心医院呼吸内科,四川遂宁629000 [3]青海大学附属医院消化内科,青海西宁810001 [4]青海省人民医院重症医学科,青海西宁810000

出　　处：《老年医学与保健》2017年第6期496-498,共3页Geriatrics & Health Care

摘　　要：目的探讨老年糖尿病肾病中NOD样受体蛋白3(NLRP3)炎性体在外周血中的表达及与肾损伤指标的关系。方法采用实时PCR技术检测60例老年糖尿病肾病患者和40例老年健康人组外周血中NLRP3炎性体基因,同时检测尿微量白蛋白、转铁蛋白、免疫球蛋白G、α1-微球蛋白和N-乙酰-β-D-氨基葡萄糖苷酶的水平。分析NLRP3炎性体与肾损伤指标之间的相关性。结果与老年健康组比较,NLRP3、凋亡相关斑点样蛋白(ASC)以及Caspase-1在老年糖尿病肾病组中表达均明显升高(均P<0.05);与老年健康组比较,早期肾损伤指标尿微量白蛋白(mAlb)、转铁蛋白(TRF)、免疫球蛋白(IgG)、α1-微球蛋白(α1-MG)和N-乙酰基β-D-葡萄糖苷酶(NAG)在老年糖尿病肾病组中表达均明显升高(P<0.05)。60例糖尿病肾病患者的NLRP3与mAlb呈正相关(r=0.880,P<0.05)、与TRF呈中度正相关(r=0.560,P<0.05)、与1-MG呈低度正相关(r=0.332,P<0.05)。结论 NLRP3炎性体可能与老年糖尿病患者肾损伤密切相关,为老年糖尿病患者肾损伤的诊断提供新依据。Objective To explore the expression of the NOD-like receptor3(NLRP3)inflammasome in peripheral blood and its correlation to renal injury indicators in the elderly with diabetic nephropathy.Methods Real-time PCR was applied in detecting NLRP3gene in60elderly with diabetic nephropathy(DN group)and40healthy elderly(norm group),and the contents of urine microalbumin(mAlb),transferring(TRF),immunoglobulinsG(IgG),alpha-1-microglobulin(α1-MG)and N-acetyl-β-D-glucosaminidase(NAGase)were detected as well.Results The expression levels of NLRP3,apoptosis-associated speck-like protein containing CARD(ASC)and Caspase-1in DN group were much higher than those in norm group(P<0.05)and the expressions of early renal injury indicators including urine mAlb,TRF,IgG,α1-MG and NAGase in the elderly in DN group increased much more significantly than those in the elderly in norm group(P<0.05);NLRP3of the60elderly in DNgroup was in positive correlation to mAlb(r=0.880,P<0.05),in moderate positive correlation to TRF(r=0.560,P<0.05)and in slight positive correlation toα1-MG(r=0.332,P<0.05).Conclusions NLRP3inflammasome may be in close correlation with renal injury indicators of the elderly with diabetic nephropathy,which provides a new basis for the diagnosis of renal injury of elderly diabetic nephropathy.

关 键 词：老年 糖尿病肾病 NOD样受体蛋白3 

分 类 号：R587.2[医药卫生—内分泌] R692.9[医药卫生—内科学]