滞流工况下管网水中微生物群落对铁释放的影响  

Influence of microbial community on iron release under stagnation condition in drinking water systems

作　　者：叶萍[1] 申屠华斌 陈环宇 李杭加 徐兵[1] 张逸夫 王磊[5] 柳景青[2] YE Ping;SHENTU Hua-bin;CHEN Huan-yu;LI Hang-jia;XU Bing;ZHANG Yi-fu;WANG Lei;LIU Jing-qing(Jiayuan Water Supply and Sewerage Company, Jiaxing 314000, China;College of Engineering and Architecture, Zhejiang University, Hangzhou 310058, China;Shanghai Water Engineering Design & Research Institute Co. Ltd, Shanghai 200063, China;Binhai Industrial Technology Research Institute of Zhejiang University, Tianjin 300301, China;College of Engineering and Architecture, Zhejiang University of Technology, Hangzhou 310014, China)

机构地区：[1]嘉兴市嘉源给排水有限公司,浙江嘉兴314000 [2]浙江大学土木工程学系,浙江杭州310058 [3]上海市水利工程设计研究院有限公司,上海200063 [4]浙江大学滨海产业技术研究院,天津300301 [5]浙江工业大学建筑工程学院,浙江杭州310014

出　　处：《中国环境科学》2017年第12期4578-4584,共7页China Environmental Science

基　　金：国家自然科学基金面上资助项目(51378455/51678520);水体污染控制与治理国家科技重大专项资助目(2012ZX07403-003)

摘　　要：为探究不同供水管道滞流过程中,管网水细菌数量和微生物群落结构对铁释放影响作用,研究依托搭载于实际供水管网中的试验平台,分别采用传统R2A培养法计数可培养腐蚀细菌数量,和高通量测序分析水中微生物群落结构.结果显示,滞流过程中不同管材管网水微生物群落以变形菌门(Proteobacteria)为主,占到86.69%~91.36%.在纲水平上,a-变形菌纲(Alphaproteobacteria)、β-变形菌纲(Betaproteobacteria)和放线菌纲(Actinobacteria)含量较高,可达50%以上.总铁浓度与铁氧化细菌和硫酸盐还原菌的相关性较强,且球墨铸铁管管网水中腐蚀细菌对总铁释放的影响性相较于HDPE管的要大.通过相关性系数分析和RDA分析,门水平上Proteobacteria、Actinobacteria和Acidobacteria以及纲水平上的Alphaproteobacteria、Betaprotebacteria和Bacilli对铁释放起到促进作用.To investigate the influence of total bacteria and microbial community structure on the iron release in water supply pipes under stagnation condition,the research was carried on the pilot platform constructed in actual water supply system.The number of culturable corrosive bacteria was counted by conventional R2A cultural method,and microbial community structure was analyzed using high-throughput sequencing(454pyrosequencing).Results show that Proteobacteria,which account for86.69%~91.36%in pipe biofilms under stagnation condition,are the dominant microbial community in phylum level.In class level,the content of Alphaproteobacteria,Betaproteobacteria and Actinobacteria excesses half of the total amount.The total iron concentration and iron oxidizing bacteria had a strong correlation with sulfate reducing bacteria.In ductile cast iron pipes,the influence of corrosive bacteria on total iron release is greater than that of HDPE pipes.By correlation coefficient and RDA analysis,it is observed that Proteobacteria,Actinobacteria and Acidobacteria in phylum level and Alphaproteobacteria,Betaproteobacteria and Bacilli in class level promote iron release.

关 键 词：供水管道 管壁生物膜 滞流工况 微生物菌落 

分 类 号：X824[环境科学与工程—环境工程]