孕酮对兔脊髓急性缺血再灌注损伤后细胞凋亡的影响  

Effect of progesterone on cells apoptosis of spinal cord after acute ischemia reperfusion injury in rabbits

作　　者：李婵 李云 靳子娟 陶柏妮 杨鹏 LI Chan;LI Yun;JIN Zijuan;TAO Bo-ni;YANG Peng(Clinical Medical School, North China University of Sciences and Technology, Tangshan 063000, China;Public Health School, North China University of Sciences and Technology, Tangshan 063000,China;Department of Neurosurgery, Affiliated Hospital, North China University of Sciences and Technology, Tangshan 063000, China)

机构地区：[1]华北理工大学临床医学院,河北唐山063210 [2]华北理工大学公共卫生学院,河北唐山063210 [3]华北理工大学附属医院神经外科,河北唐山063210

出　　处：《中国临床神经外科杂志》2017年第11期767-770,共4页Chinese Journal of Clinical Neurosurgery

基　　金：河北省医学科学研究重点课题计划(ZD20140046);华北理工大学大学生创新创业训练计划(X2016093)

摘　　要：目的探讨孕酮对兔脊髓急性缺血再灌注损伤后细胞凋亡的影响。方法将90只日本大耳白兔随机分为假手术组(n=30)、脊髓损伤组(n=30)与孕酮治疗组(n=30),各组又进一步分为12 h、24 h、36 h、48 h、72 h、14 d等6个亚组,每亚组5只。假手术组只行腹部切开术但不阻断腹主动脉。脊髓损伤组和孕酮治疗组均制作缺血再灌注模型,制模成功后孕酮治疗组每24h注射孕酮1次(将孕酮溶于玉米油,浓度10 mg/ml,注射剂量为8 ml/kg),脊髓损伤组在相同时间点注射等量生理盐水。采用TUNEl染色评估细胞凋亡,采用BBB评分评估运动功能。结果 HE染色结果显示,假手术组脊髓形态正常;脊髓损伤组可见脊髓结构被破坏,正常神经元数目减少;孕酮治疗组较脊髓损伤组正常神经元数目多。TUNEL染色结果显示,脊髓损伤组和孕酮治疗组各时间点凋亡细胞数均明显高于假手术组(P<0.05);孕酮治疗组各时间点凋亡细胞的数目均明显少于脊髓损伤组(P<0.05)。假手术组术后12 h BBB评分为17.3分,术后14 d时升高到21.0分;脊髓损伤组术后12 h BBB评分为0.6分,随后缓慢升高,术后14 d时评分为9.2分。孕酮治疗组BBB评分变化与脊髓损伤组相似,但术后36 h开始,各时间点评分均明显高于相应脊髓损伤组(P<0.05)。结论孕酮治疗可改善脊髓急性缺血再灌注损伤兔运动功能,可能与抑制细胞凋亡有关。Objective To explore effect of progesterone on the cells apoptosis of spinal cord after acute ischemia reperfusion injury in rabbits.Methods Ninety rabbits were randomly divided into sham operation group(n=30),spinal cord injury(SCI)group(n=30)and progesterone treatment group(n=30).The cell apoptosis in the injured spinal cord and the movement function of posterior limbs were determined respectively by terminal deoxynucleotidayl transferase dUTP nick end labeling and Basso,Beattie&Bresnahan(BBB)locomotor rating scale12,24,36,48,72and336hours after the injury in all the animals.Results The apoptotic cells were observed in the injured spinal cord12hours after SCI,and the apoptosis peaked48hours after SCI and apoptotic cells were still observed14days after SCI.The number of apoptosis cells was significantly fewer in progesterone treatment group than that in SCI group36,48,72and336hours after SCI(P<0.05).The BBB locomotor rating scale scores were significantly higher in progesterone treatment group than those in SCI group36,48,72and336hours after SCI(P<0.05).Conclusion It is suggested that progesterone may play a neuroprotective effect by inhibiting apoptosis in the rabbits with acute ischemia-reperfusion injury.

关 键 词：脊髓缺血再灌注损伤 孕酮 细胞凋亡 日本大耳白兔 

分 类 号：R651.2[医药卫生—外科学]