Characterization and evaluation of rice blast resistance of Chinese indica hybrid rice parental lines  

作　　者：Yunyu Wu Ling Yu Ning Xiao Zhengyuan Dai Yuhong Li Cunhong Pan Xiaoxiang Zhang Guangqing Liu Aihong Li 

机构地区：[1]Lixiahe Agricultural Research Institute of Jiangsu Province, Yangzhou 225009, China [2]Jiangsu Collaborative Innovation Center for Modern Crop Production, Nanjing 225009, China [3]Jiangsu Golden Land Seed Industry Co., Ltd, China

出　　处：《The Crop Journal》2017年第6期509-517,共9页作物学报（英文版）

基　　金：supported by the Key Research and Development Project of Jiangsu Province for Modern Agriculture (BE2015336);the "333" Project of Jiangsu Province, China (BRA2016145);the Natural Science Foundation for Young Scientists of Jiangsu Province, China (BK20160447);the Natural Science Foundation for Young Scientists of Yangzhou, Jiangsu Province, China (YZ2016103);Yangzhou international technology cooperation project (YZ2015145)

摘　　要：The development of resistant varieties and hybrid combinations has been the most effective and economical strategy to control blast disease caused by Magnaporthe oryzae.However, the distribution of major R genes and blast resistance characterization in hybrid rice parents has not been well investigated, resulting in their limited use in hybrid rice blast-resistance breeding. In the present study, 88 elite indica hybrid rice parental lines were evaluated with 30 isolates of M. oryzae collected from the main planting area of indica hybrid rice in China and were characterized for the presence of 11 major resistance genes using molecular markers. The pathogenicity assays showed that four types of hybrid rice parent line showed some resistance to M. oryzae. However, the proportions of highly resistant lines and the mean resistance frequency(RF) varied among the four types, with resistance in decreasing order shown by three-line restorer lines, three-line maintainer lines, two-line sterile lines, and two-line restorer lines. All 88 hybrid rice parental lines carried more than one R gene, but none carried the R genes Pi1 and Pi2. Although Pid3 and Pi9 were present only in three-line restorer lines and Pigm only in three-line maintainer lines, the remaining six R genes(Pib, Pid2, Pi5, Pia, Pi54, and Pita) were present in the four types of hybrid rice parent with significantly different distribution frequencies. The correlation between R genes and resistance reactions was investigated. The results are expected to provide useful information for rational utilization of major R genes in hybrid rice breeding programs.The development of resistant varieties and hybrid combinations has been the most effective and economical strategy to control blast disease caused by Magnaporthe oryzae.However, the distribution of major R genes and blast resistance characterization in hybrid rice parents has not been well investigated, resulting in their limited use in hybrid rice blast-resistance breeding. In the present study, 88 elite indica hybrid rice parental lines were evaluated with 30 isolates of M. oryzae collected from the main planting area of indica hybrid rice in China and were characterized for the presence of 11 major resistance genes using molecular markers. The pathogenicity assays showed that four types of hybrid rice parent line showed some resistance to M. oryzae. However, the proportions of highly resistant lines and the mean resistance frequency(RF) varied among the four types, with resistance in decreasing order shown by three-line restorer lines, three-line maintainer lines, two-line sterile lines, and two-line restorer lines. All 88 hybrid rice parental lines carried more than one R gene, but none carried the R genes Pi1 and Pi2. Although Pid3 and Pi9 were present only in three-line restorer lines and Pigm only in three-line maintainer lines, the remaining six R genes(Pib, Pid2, Pi5, Pia, Pi54, and Pita) were present in the four types of hybrid rice parent with significantly different distribution frequencies. The correlation between R genes and resistance reactions was investigated. The results are expected to provide useful information for rational utilization of major R genes in hybrid rice breeding programs.

关 键 词：Hybrid rice PARENTAL lines MAGNAPORTHE ORYZAE Pi genes RESISTANCE EVALUATION Molecular markers 

分 类 号：S435.111.41[农业科学—农业昆虫与害虫防治]