维拉帕米及艾灸对长春新碱耐药胃癌细胞株SGC7901逆转增效作用  

Reversaland synergisticeffect of verapamil and moxibustion on vincristine resistance in mice with SGC7901 gastric cancer

作　　者：王楠[1] 宋小骏[1] 蒋凤[1] 谢学建[1] WANG Nan;SONG Xiao-jun;JIANG Feng;XIE Xue-jian(Department of Pharmacy,Nanjing General Hospital of Nanjing Military Region, PLA,Nanjing 210002,Jiangsu,China)

机构地区：[1]南京军区南京总医院药品科,南京210002

出　　处：《东南国防医药》2017年第6期587-591,共5页Military Medical Journal of Southeast China

基　　金：江苏省药学会-奥赛康医院药学科研基金项目(201201)

摘　　要：目的观察维拉帕米(VPM)及艾灸对小鼠长春新碱(VCR)耐药SGC7901胃癌移植后的瘤体重量、抑瘤率及P-糖蛋白(P-gp)表达的影响,以了解两者对长春新碱耐药逆转增效作用。方法分别进行不同剂量维拉帕米及艾灸联合不同剂量维拉帕米抗肿瘤耐药的实验。第一组实验将SD小鼠分成等渗盐水组、VCR对照组、VPM对照组、0.5 mg/kg VPM+VCR组、1.0 mg/kg VPM+VCR组、1.5 mg/kg VPM+VCR组、2.0 mg/kg VPM+VCR组,,每组16只,雌雄各半,所有模型组小鼠在左前肢腋下接种瘤组织,按组给予等渗盐水和不同剂量药物,15 d后,取出瘤体称重,计算抑瘤率,同时留取瘤体标本,检测多药耐药(MDR)相关蛋白P-gp的变化。第二组实验将SD小鼠分成等渗盐水组、VCR对照组、VPM对照组、艾灸对照组、0.5 mg/kg VPM+VCR+艾灸组、1.0 mg/kg VPM+VCR+艾灸组、1.5 mg/kg VPM+VCR+艾灸组、2.0 mg/kg VPM+VCR+艾灸组,每组16只,雌雄各半,所有模型组小鼠在左前肢腋下接种瘤组织,按组给予等渗盐水和不同剂量药物及艾灸,15 d后,取出瘤体称重,计算抑瘤率,同时留取瘤体标本,检测MDR相关蛋白P-gp的变化。结果第一组实验中给予维拉帕米时,等渗盐水组与不同剂量药物组间差异有统计学意义(P<0.05),随剂量增加瘤体重量下降、抑瘤率增加和蛋白P-gp表达下降,但1.5mg/kg和2.0 mg/kg时,蛋白P-gp值差异无统计学意义。第二组实验中给予维拉帕米和艾灸时,等渗盐水组与不同剂量药物组间差异也有统计学意义(P<0.05),随剂量增加瘤体重量下降、抑瘤率增加和蛋白P-gp表达下降,其中剂量组:1.5 mg/kg和2.0 mg/kg与仅给予维拉帕米组相比差异有统计学意义(P<0.05)。结论维拉帕米和艾灸对小鼠长春新碱耐药SGC7901胃癌耐药具有逆转增效作用,在2.0 mg/kg维拉帕米、0.5 mg/kg长春新碱、艾灸联合用药时可达到单用1.0 mg/kg长春新碱用药效果。ObjectiveThe purpose of this study is to investigate the effect of verapamil and moxibustion on the tumor inhibitory rate and the tumor weight and the expression of P-glycoprotein after the transplantation of vincristine(VCR)resistant SGC7901gastric cancer in mice,so as to understand the reversal effect of these two drugs on vincristine resistance.MethodsIn first group SD mice were divided into normal saline group,vincristine control group,Verapamil control group,No1drug group,No2drug group,No3drug group,No4drug group,16rats in each group,Half and half on male and female,all mice inoculated with tumor tissue in the left forelimb,and administered with different doses of drugs and moxibustion.On15days later,inhibition rate of the tumorwas calculated.At the same time for tumor specimens,changes of MDR related protein P-gp are detected.In second group SD mice were divided into normal saline group,vincristine control group,Verapamilcontrol group,Moxibustion group,No1drug+Moxibustion group,No2drug+Moxibustion group,No3drug+Moxibustion group,No4+Moxibustion drug group,16rats in each group,Half and half on male and female,all mice inoculated with tumor tissue in the left forelimb,and administered with different doses of drugs and moxibustion.On15days later,inhibition rate of the tumor was calculated.At the same time for tumor specimens,changes of MDR related protein P-gp are detected.ResultsOnly to give the Verapamil treatment,there was significant difference between the Saline group and different dose groups(P<0.05),the tumor inhibition rate increased and the tumor weight and protein P-gp decreased along with the increase of the drug dose;At the1.5mg/kg and2.0mg/kg,there was no significant difference in protein P-gp.To give Verapamil and Moxibustion treatment,there is also a significant difference in the saline group and different dose groups(P<0.05),and the tumor inhibition rate increased and the tumor weight and protein P-gp decreased along with the increase of the dose.At the dose group(1.5mg/kg and2.0mg/kg)there wer

关 键 词：维拉帕米 艾灸 SGC7901胃癌 长春新碱 耐药 逆转增效 

分 类 号：R9[医药卫生—药学]