离子液体-中空纤维膜液相微萃取-高效液相色谱法快速测定卷烟烟气中的苯并[a]芘  

Hollow Fiber Liquid-Phase Micro-Extraction Method Based on Ionic Liquids Followed by High-Performance Liquid Chromatography For Rapid Determination of Benzo(A) Pyrene in Cigarette Smoke

作　　者：刘春波 缪明明 刘志华 王蒙[2] 张磊 杨亚玲[2] LIU Chun-bo;MIAO Ming-ming;LIU Zhi-hua;WANG Meng;ZHANG Lei;YANG Ya-ling(Key Lab of Tobacco Chemistry of Yunnan,Technology Center of Yunnan Tobacco Industry Limited Liability Company,Kunming 650231,China;Faculty of Life Science and Technology,Kunming University of Science and Technology,Kunming 650500,China;Yunnan Jianniu Biotechnology Co.,Ltd,Kunming 650033,China)

机构地区：[1]云南省烟草化学重点实验室,云南中烟工业有限责任公司技术中心,云南昆明650231 [2]昆明理工大学生命科学与技术学院,云南昆明650500 [3]云南健牛生物科技有限公司,云南昆明650033

出　　处：《云南师范大学学报（自然科学版）》2017年第6期54-59,共6页Journal of Yunnan Normal University:Natural Sciences Edition

基　　金：国家自然科学基金资助项目(21365026)

摘　　要：将中空纤维膜液相微萃取技术与高效液相色谱-荧光检测器相结合,建立卷烟烟气中苯并[a]芘的分析检测新方法.以疏水性离子液体1-辛基-3-甲基咪唑六氟磷酸盐([OMIM]PF6)作萃取剂,十一烷酸乙腈溶液作为液膜支持相溶剂,在最佳实验条件下,萃取平衡可在3 min内完成.苯并[a]芘浓度在0.1~20.0ng/mL范围内与其色谱峰面积呈良好的线性关系,相关系数R2=0.999 4,检出限为0.05ng/mL.该方法已成功应用于卷烟烟气中苯并[a]芘的分析检测,回收率为93.6%~102.4%,相对标准偏差为2.7%~4.8%.A rapid,simple,reliable and novel hollow fiber liquid-phase micro-extraction method based on ionic liquids(IL-HF-LPME)was successfully developed for determination of benzo(a)pyrene(BaP)in cigarette smoke samples followed by high-performance liquid chromatography-fluorescence detection(HPLC-FLD).Inthe IL-HF-LPME method,hydrophobic ionic liquid1-octyl-3-methylimidazolium hexafluorophosphate([OMIM]PF6)was used as the extraction solvent,and the undecanoic acid acetonitrile solution was used as the supported liquid membrane phase.Under the optimum experimental conditions,the extraction equilibrium can be completed within3min.A good linearity for the analysis of benzo(a)pyrene in the concentration range of0.1~20.0ng/mL was obtained with acorrelation coefficient(R2)of0.9994,and the limit of detection(LOD)was0.05ng/mL.The developed method has been successfully applied for analysis of benzo(a)pyrene in cigarette smoke samples.The recovery were93.6%~102.4%,and the relative standard deviation was2.7%~4.8%.

关 键 词：苯并[A]芘 离子液体 中空纤维膜液相微萃取 高效液相色谱-荧光检测器 卷烟烟气 

分 类 号：TS47[农业科学—烟草工业]