PPRV中国新疆南疆株F基因遗传演化分析  

Genetic Evolution Analysis of F gene of PPRV in Southern Xinjiang

作　　者：张路瑶 李飞[2,3] 焦海宏[2] 赵丽 刘永宏[2] ZHANG Lu-yao;LI Fei;JIAO Hai-hong;ZHAO Li;LIU Yong-hong(nimal Husbandry and Veterinary Workstations of Barkol Kazak Autonomous County, Kumul Xinjiang 839200, China;College of Animal Science, Tarim University, Key Laboratory of Tarim Animanl Husbandry Science and Technology of Xinjiang Production & Construction Corps, Alar Xinjiang 843300 China;Aksu Animal Loimia Controlling and Diagnostic Center, Aksu Xinjiang 843300, China)

机构地区：[1]巴里坤哈萨克自治县畜牧兽医工作站,新疆哈密839200 [2]塔里木大学动物科学学院/新疆生产建设兵团塔里木畜牧科技重点实验室,新疆阿拉尔843300 [3]新疆阿克苏地区动物疫病控制诊断中心,新疆阿克苏843000

出　　处：《新疆农业科学》2017年第11期2118-2129,共12页Xinjiang Agricultural Sciences

基　　金：华中农业大学塔里木大学科研联合基金"新疆南疆小反刍兽疫流行病学调查"(HNTDLH1404)~~

摘　　要：【目的】分析中国新疆南疆2014年PPRV毒株来源,为中国新疆南疆PPR防控提供理论依据。【方法】调查中国新疆南疆阿克苏地区某羊场发病羊发病情况、临床症状观察及病理学检查,实验室进行PPRV F基因RT-PCR和F基因测序,进一步对PPRV F基因序列分析。【结果】毒株与国内PPRV毒株F基因核苷酸同源性最高的是中国新疆China/XJYL/2013毒株,与国外的毒株F基因核苷酸同源性最高的是印度Izatnagar/94毒株及Izatnagar-94毒株;此次毒株与国内PPRV毒株F蛋白氨基酸同源性最高的是中国新疆China/XJYL/2013毒株,与国外的PPRV毒株F蛋白氨基酸同源性最高的是印度Izatnagar/94毒株。PPRV毒株F基因进化树分析显示:PPRV毒株与北京PPRV毒株进化关系最近,与中国新疆伊犁PPRV毒株同属一个分支,与国外印度及孟加拉国毒株的进化关系较近。【结论】2014年中国新疆南疆存在PPR疫情,该疫情毒株与此次疫情前后国内的PPR毒株及印度PPR毒株进化关系较近。【Objective】In order to provide a theoretical basis for the prevention and control of PPR in southern Xinjiang,the PPRV strains in2014was analyzed.【Method】The incidence of sheep in a sheep farm in Akesu Prefecture of southern Xinjiang was investigated.The clinical symptoms and pathological examination were performed.The PPRV F gene RT-PCR and F gene sequencing were carried out in the laboratory,and the sequence analysis of PPRV F gene was further carried out.【Result】PPRV F gene sequence analysis showed that the test China/XJYL/2013in Xinjiang had the highest homology with domestic PPRV strainF gene nucleotide.Compared with the Foreign strains,the highest homology of nucleotide is India strains Izatnagar/94and Izatnagar-94strains;PPRV strain F gene phylogenetic tree analysis in this study showed that the relationship of this PPRV strain had recently evolved from Beijing PPRV strain and belonged to the same branch of Ili PPRV strain,which had close evolutionary relationship with foreign strains in India and Bangladesh.【Conclusion】There existed PPR epidemic in southern Xinjiang in2014,and the epidemic strains and the domestic PPR before and after the outbreak of the PPR strains and the surrounding countries strains were relatively close to the evolution.

关 键 词：小反刍兽疫 诊断 F基因 序列分析 

分 类 号：S852.6[农业科学—基础兽医学]