利用BSR-Seq定位小麦品种郑麦103抗条锈病基因YrZM103  

Mapping Stripe Rust Resistance Gene YrZM103 in Wheat Cultivar Zhengmai 103 by BSR-Seq

作　　者：张怀志 谢菁忠 陈永兴 刘旭[3] 王勇[1] 闫素红[3] 杨兆生[3] 赵虹[4] 王西成[4] 贾联合 曹廷杰[4] 刘志勇 ZHANG Huai-Zhi;XIE Jing-Zhong;CHEN Yong-Xing;LIU Xu;WANG Yong;YAN Su-Hong;YANG Zhao-Sheng;ZHAO Hong;WANG Xi-Cheng;JIA Lian-He;CAO Ting-Jie;LIU Zhi-Yong(College of Agronomy & Biotechnology, China Agricultural University, Beijing 100193, China;State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China;Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang 455000, China;Wheat Research Institute, Henan Academy of Agricultural Sciences Zhengzhou 450002, China;Tangxian Vocational and Technical Educational Center, Tangxian 072350, China)

机构地区：[1]中国农业大学农学院,北京100193 [2]中国科学院遗传与发育生物学研究所,北京100101 [3]中国农业科学院棉花研究所,河南安阳455000 [4]河南省农业科学院小麦研究所,河南郑州450002 [5]河北省唐县职业技术教育中心,河北唐县072350

出　　处：《作物学报》2017年第11期1643-1649,共7页Acta Agronomica Sinica

基　　金：国家重点研发计划项目(2017YFD0101802)资助~~

摘　　要：郑麦103是一个高抗条锈病的小麦新品种,为明确其携带的抗病基因,用郑麦103与感条锈病品种农大399杂交构建分离群体,用条锈菌CYR32、CYR33和CRY34(V26)混合菌系进行田间接种和成株期抗性鉴定,对214个F2:3家系的条锈病抗性进行遗传分析,初步确定郑麦103的抗条锈性由单个主效基因控制,定名为Yr ZM103。通过BSR-Seq技术开发了6个与Yr ZM103紧密连锁的分子标记,将Yr ZM103定位于染色体臂7BL分子标记ZM215和ZM221之间,遗传距离分别为11.8 c M和6.9 c M。利用7BL染色体上与其他已知抗条锈病基因紧密连锁的分子标记进行比较作图,发现Yr ZM103是不同于7BL末端其他抗条锈病基因的新基因。Zhengmai103is a new wheat cultivar highly resistant to stripe rust.In order to detect the stripe rust resistance gene in Zhengmai103,a segregating population was developed by making cross between Zhengmai103and a highly stripe rust susceptible wheat cultivar Nongda399.The214F2derived F2:3progenies were inoculated with mixed prevailing Pst races CYR32,CYR33and CYR34(V26)in field condition for disease resistance evaluation at adult plant-stage.Genetic analysis revealed that the stripe rust resistance of Zhengmai103is controlled by a single gene,temporarily designated YrZM103.By applying RNA-seq with bulked segregant analysis(BSA),six polymorphic markers were developed to map YrZM103on chromosome7BL flanked by markers ZM215and ZM221with genetic distances of6.9cM and11.8cM,respectively.Comparative genetic mapping indicated YrZM103was located on different genetic interval from that of known stripe rust resistance genes on7BL.

关 键 词：郑麦103 条锈病 分子标记 BSR-Seq 小麦 

分 类 号：S435.121.42[农业科学—农业昆虫与害虫防治]