外源水杨酸、脯氨酸和γ-氨基丁酸对盐胁迫下水稻产量的影响  

Effect of Exogenous Salicylic Acid, Proline, and γ-Aminobutyric Acid on Yield of Rice under Salt Stress

作　　者：沙汉景[1] 胡文成 贾琰[1] 王新鹏[1] 田雪飞[1] 于美芳[1] 赵宏伟[1] SHA Han-Jing;HU Wen-Cheng;JIA Yan;WANG Xin-Peng;TIAN Xue-Fei;YU Mei-Fang;ZHAO Hong-Wei(Rice Research Institute, Northeast Agricultural University, Harbin 150030, China)

机构地区：[1]东北农业大学水稻研究所,黑龙江哈尔滨150030

出　　处：《作物学报》2017年第11期1677-1688,共12页Acta Agronomica Sinica

基　　金：国家重点研发计划项目(2016YFD0300104);黑龙江省重大科技招标项目(GA14B102-02)资助~~

摘　　要：水杨酸(SA)、脯氨酸(Pro)和γ-氨基丁酸(GABA)在植物生长发育以及抵御逆境胁迫中起重要作用。为了研究三者复配对盐胁迫下水稻产量是否具有协同增效作用,本文采用三元二次通用旋转组合设计,建立了SA、Pro和GABA复配剂与盐胁迫下水稻产量之间的数学模型,通过频数分析获得SA、Pro与GABA复配优化组合方案。结果表明,二次模型拟合较好,对盐胁迫下水稻产量的影响程度为SA>GABA>Pro,SA与GABA,Pro与GABA之间交互作用对盐胁迫下水稻产量有显著影响。模拟寻优获得提高盐胁迫下水稻产量10%以上的最佳复配组合为SA 0.44~0.51mmol L–1、Pro 27.63~31.20 mmol L–1、GABA 3.55~4.28 mmol L–1。外源SA、Pro与GABA单剂和复配剂对盐敏感品种牡丹江30的调控作用大于耐盐品种龙稻5号。SA或Pro与GABA复配对盐胁迫下水稻产量的影响具有协同作用,而SA与Pro复配效果与品种耐盐性差异有关。SA、Pro和GABA三者复配效果优于两两复配,任意单剂与其他2种外源物质复配对水稻产量的影响均存在协同作用。Salicylic acid(SA),proline(Pro),andγ-aminobutyric acid(GABA)play important roles in plant growth and development and tolerating stress.In order to investigate whether the combination of SA,Pro,and GABA had synergistic effect on the yield of rice under salt stress,the mathematical models were established by means of quadratic general rotary unitized design.The optimal combination of SA,Pro,and GABA was obtained by frequency analysis.The results demonstrated that the quadratic models fit better.The effects on the yield of rice under salt stress were SA>GABA>Pro.The interactions between SA and GABA,and between Pro and GABA showed significant effects on rice yield under salt stress.The recommended optimal combination is0.44?0.51mmol L-1for SA,27.63?31.20mmol L-1for Pro and3.55?4.28mmol L-1for GABA,which can improve the rice yield more than10%under salt stress.The effect of a single or a combination of SA,Pro,and GABA on the yield of salt-sensitive variety MDJ30was larger than that of salt-tolerant variety LD5.The combination of SA and GABA,or Pro and GABA showed synergistic effect on the yield of rice under salt stress,while the combination effect of SA and Pro,was related to the genotype difference in salt tolerance.The joint application of SA,Pro,and GABA showed better effect on the yield of rice under salt stress than that of any two of them.The combination between any single agent and the other two agents showed synergisticeffect.

关 键 词：二次通用旋转组合设计 协同作用 盐胁迫 水稻 产量 

分 类 号：S511[农业科学—作物学]