高效液相色谱法测定西布曲明、麻黄碱和芬氟拉明试验  

Experiment on Determination of Sibutramine, Ephedrine and fenfluramine by HPLC

作　　者：曹宁阳 董娇 吴迪 王烁 岑琴[2] CAO Ningyang;DONG Jiao;WU Di;WANG Shuo;CEN Qin(Instrument Room Shenyang Institute for Food Control, Shenyang 110136, China;Shenyang Product Quality Supervision and Inspection Institute, Shenyang 110022, China)

机构地区：[1]沈阳食品检验研究所仪器室,沈阳110136 [2]沈阳产品质量监督检验院,沈阳110022

出　　处：《农业科技与装备》2017年第5期52-54,共3页Agricultural Science & Technology and Equipment

摘　　要：为建立高效液相色谱同时测定减肥类保健品中非法添加药物西布曲明、麻黄碱和芬氟拉明的方法,样品经液相色谱分离后采用二极管阵列检测器检测,以保留时间和紫外特征吸收光谱图作为定性依据。试验结果表明:西布曲明、麻黄碱、芬氟拉明的检出限分别为20,40,15μg/mg;在4~80μg/mL浓度范围,线性相关系数均大于0.999 5;样品加标回收率大于95%,RSD小于3.4%。To establish HPLC and the method for determination of sibutramine,ephedrine and fenfluramine illegally added in slimming food,the sample was separated by liquid chromatography,then detected by photodiode array detector,so as to retention time and the ultraviolet characteristic absorption spectrum as basis for determining the nature.The experimental results showed that:The detection limits of sibutramine,ephedrine and fenfluramine were20,40and15μg/mg;Whenconcentration range was from4to80μg/mL,the linearly dependent coefficients were all above0.9995;The adding standard recovery was above95%,and RSD<3.4%.

关 键 词：高效液相色谱法 西布曲明 麻黄碱 芬氟拉明 标准曲线 

分 类 号：TS218[轻工技术与工程—粮食、油脂及植物蛋白工程]