桃叶杜鹃菌根苗对干旱胁迫的生理响应  

Physiological responses of mycorrhizal Rhododendron annae seedlings to drought stress

作　　者：陈荣建 欧静[1] 王丽娟[1] 龙海燕[1] 熊贤荣 熊丹 CHEN Rongjian;OU Jing;WANG Lijuan;LONG Haiyan;XIONG Xianrong;XIONG Dan(College of Forestry, Guizhou University, Guiyang 550025, China)

机构地区：[1]贵州大学林学院,贵州贵阳550025

出　　处：《湖南农业大学学报（自然科学版）》2017年第6期635-639,共5页Journal of Hunan Agricultural University(Natural Sciences)

基　　金：国家自然科学基金项目(31560223);贵州省科技计划项目(黔科合支撑[2016]2522号);贵州大学研究生创新基金项目(研农2016014)

摘　　要：以桃叶杜鹃为材料,接种4种(TY02、TY21、TY29、TY35)杜鹃花类菌根(ERM)真菌,以不接种处理为对照(CK),进行干旱胁迫,之后再复水,研究接种ERM真菌桃叶杜鹃的叶片相对含水量、渗透调节物质、SOD活性、MDA含量、光合色素等生理生化指标的变化。结果表明:接种ERM真菌能有效提高桃叶杜鹃叶片相对含水量,降低叶片水分饱和亏缺,在第15天接种TY29处理桃叶杜鹃叶片的含水量较对照增加了91.62%,复水后,接种TY35处理桃叶杜鹃叶片相对含水量最高,较对照提高了154.6%;接种ERM能显著提高桃叶杜鹃的可溶性糖、可溶蛋白、游离脯氨酸含量;接种ERM真菌能显著提高桃叶杜鹃的SOD活性,以接种TY35处理在第10天的SOD活性最大,较对照组增加69.4%;接种ERM真菌能显著降低MDA含量。在干旱胁迫下,接种ERM真菌能提高桃叶杜鹃叶片的相对含水量、可溶性糖、可溶性蛋白、脯氨酸含量、SOD活性、光合色素的含量,降低MDA含量,从而提高桃叶杜鹃幼苗的抗旱能力,其中以接种TY29和TY35的效果较好。To research the physiological responses to drought stress of mycorrhizal seedlings of Rhododendron annae mycorthiza,its seeds were inoculated with four ERM fungi(TY02,TY21,TY29,TY35)respectively,with non–inoculation as the control,and the relative water content,water saturation deficit,the adjustment of osmotic substances(soluble sugar,soluble protein,proline),the enzymatic activity of SOD,MDA content,the physiologic index and biochemical criterion of photosynthetic pigment of Rhododendron annae leaves were measured under drought stress.The results showed that inoculation of ERM fungi could improve leaves relative water content and reduce water saturation deficit.In the15th day after inoculated with TY29,the relative water content of the leaves was increased by91.62%;after re–watering,the relative water content of the leaves treated with TY35was154.6%higher than that of the control group.Inoculation with ERM fungi could significantly increase the soluble sugar,proline,soluble protein and other osmotic adjustment substances of Rhododendron annae.Inoculation with ERM fungi could enhance the adaptability of Rhododendron annae to drought stress by increasing SOD activity and reducing the MDA content.In the10th day,SOD activity inoculated with TY35was the best,it was69.4%higher than that of control group.In conclusion,the abilities of drought resistance inoculated with TY29and TY35were better than those of the others.

关 键 词：桃叶杜鹃 杜鹃花类菌根(ERM) 干旱 生理响应 

分 类 号：S685.21[农业科学—观赏园艺]