黄芩素延缓大鼠心肌衰老的作用及机制  

Myocardial Protective Effect of Baicalein on Aging Rats Induced by D-galactose

作　　者：李爽[1] 刘璐[1] 胡宝荣[1] 张婧[1] 宋永熙[1] 海鑫 LI Shuang;LIU Lu;HU Bao-rong;ZHANG Jing;SONG Yong-xi;HAI Xin(The First Affiliated Hospital of Harbin Medical University, Harbin 150001, China)

机构地区：[1]哈尔滨医科大学附属第一医院药学部,黑龙江哈尔滨150001

出　　处：《中医药学报》2017年第6期57-61,共5页Acta Chinese Medicine and Pharmacology

摘　　要：目的:研究黄芩素是否具有延缓大鼠心肌衰老的作用及机制。方法:将75只成年SD大鼠随机分为正常对照组、模型组和黄芩素高中低剂量组(200、100、50 mg/kg),正常对照组每日皮下注射生理盐水(10 mL/kg),其余4组均每日皮下注射D-半乳糖生理盐水溶液(150 mg/kg)连续30天,建立衰老模型,同时黄芩素各剂量组大鼠灌胃相应药物,正常对照组与模型组灌胃等体积生理盐水。给药30天后,采用超声心动仪对动物心脏功能进行评价,检测各组大鼠心肌组织中的超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)、丙二醛(MDA)含量。SA-β-gal活性检测试剂盒检测心肌组织β-半乳糖苷酶活性。Western blot衰老相关蛋白p21^(Cip1/Waf1)及p53表达。结果:与正常对照组比较,模型组大鼠心脏功能下降,左室射血分数、短轴缩短率降低(P<0.01);心肌组织β-半乳糖苷酶活性明显增加(P<0.01);血清与心肌中的SOD和GSH-Px下降,MDA含量增加(P<0.01),衰老相关蛋白p21^(Cip1/Waf1)及p53表达增加。与模型组比较,黄芩素组大鼠心脏功能增加,MDA含量下降,SOD和GSHPx水平升高,衰老相关蛋白p21^(Cip1/Waf1)及p53表达降低,且呈剂量相关性,高剂量组改善更明显。结论:黄芩素对大鼠心肌衰老有一定的延缓作用,其机制可能与提高衰老心肌抗氧化能力,抑制其下游p53/p21^(Cip1/Waf1)信号通路有关。Objective:To study the protective effect of baicalein on the aging model rats and explore the underlying mechanism.Methods:75rats were randomly divided into the blank control group,the model group,high-dose baicalein group(200mg/kg),medium-dose baicalein group(100mg/kg),and low-dose baicalein group(50mg/kg),with15rats in each group.The aging models were established by the injection of D-galactose solution(150mg/kg)for30d,whereas the blank control group was injected saline(10mL/kg).Different concentrations of baicalein were applied to the rats in baicalein groups for30d after modeling,at the same time,same volume of saline was given to the blank control group and the model group.Cardiac function was evaluated with Echocardiography,and SOD,GSH-Px and MDA were tested in the myocardium.SA-β-gal activity and expressions of p21Cip1/Waf1and p53were examined.Results:Compared to blank control group,in the model group,the cardiac function of aging rats was declined(P<001);the activity of SOD and GSH-Px in the myocardium were decreased significantly(P<0.01);SA-β-gal-positive cells and MDA contents in the myocardium were increased significantly(P<0.01);expressions of p53and p21Cip1/Waf1were also increased;Compared to the model group,the cardiac function in the baicalein groups was improved;the activities of SOD and GSH-Px in myocardium were increased significantly,with decreased MDA SA-β-gal-positive cells in the baicalein groups(P<0.01);the expressions of p53and p21Cip1/Waf1were also significantly decreased in baicalein groups.The anti-aging effect of baicalein was dose dependent,the high-dose group improved more than the other two groups.Conclusion:Baicalein has obvious protective effect on myocardial cells in aging rats induced by D-galactose,the mechanism may be related to the decreased oxidative stress level and inhibiting its downstream p53/p21Cip1/Waf1signaling pathway,improving heart function in aging rats.

关 键 词：黄芩素 抗衰老 大鼠 氧化损伤 心脏 

分 类 号：R285.5[医药卫生—中药学] R322.1[医药卫生—中医学]