针刺不同穴位对寒凝证类痛经大鼠子宫收缩程度及疼痛相关机制的影响  

Effect and the Mechanism of Acupuncture at Different Acupoints for the Uterus Contractions in Dysmenorrhea Rats with Cold Congealing

作　　者：杨佳敏 沈小雨[2] 张玲[2] 罗丽 许咏思[2] 任晓暄[2] 郭孟玮[2] 赵雅芳[2] 申松希[2] 朱世鹏[2] 齐丹丹[2] 嵇波[2] 李晓泓[2] 朱江[2] 张露芬[2] YANG Jia-min;SHEN Xiao-yu;ZHANG Ling;LUO Li;XU Yong-si;REN Xiao-xuan;GUO Meng-wei;ZHAO Ya-fang;SHEN Song-xi;ZHU Shi-peng;QI Dan-dan;JI bo;LI Xiao-hong;ZHU Jiang;ZHANG Lu-fen(Beijing Vocational College of Labour and Social Security, Beijing 100029, China;College of Acupuncture and Tuina, Beijing University of Chinese Medicine, Beijing 100029, China)

机构地区：[1]北京劳动保障职业学院,北京100029 [2]北京中医药大学针灸推拿学院,北京100029

出　　处：《中医药学报》2017年第6期73-76,共4页Acta Chinese Medicine and Pharmacology

基　　金：国家重点基础研究发展计划(973计划)(No.2012CB518506);国家中医药管理局针灸生物学三级实验室

摘　　要：目的:比较针刺三阴交穴、关元穴对寒凝证类痛经大鼠子宫平滑肌收缩程度及脊髓、子宫组织中κ-受体、μ-受体含量的影响,探讨不同穴位介入的效应差异及部分机制。方法:将64只雌性SD大鼠随机分为盐水组、寒凝证类痛经模型组、针刺三阴交组、针刺关元组,每组16只。采用皮下注射苯甲酸雌二醇和低温冷冻的方法制备寒凝证类痛经模型,末次给药1 h后,麻醉大鼠,运用BL-420F生物机能实验系统记录子宫的收缩程度。采用Real Time-PCR检测大鼠脊髓及子宫组织中κ-受体、μ-受体的含量。结果:1.子宫平滑肌收缩程度:与盐水组比较,模型组子宫收缩波的个数显著增加(P<0.01)、子宫收缩波峰峰值显著升高(P<0.05)、子宫活动度显著增强(P<0.01),三阴交组、关元组子宫活动度显著增强(P<0.01,P<0.05);与模型组比较,三阴交组与关元组子宫收缩波的个数及子宫活动度均显著下降(P<0.05)。2.脊髓及子宫组织中κ-受体、μ-受体水平:(1)脊髓中的κ-受体及μ-受体水平:各组间比较无统计学差异;(2)子宫组织中的κ-受体水平:各组间比较无统计学差异;(3)子宫组织中μ-受体水平:与盐水组比较,关元组子宫组织中μ-受体水平显著升高(P<0.05);与模型组比较,三阴交组、关元组子宫组织中μ-受体水平均显著升高(P<0.05,P<0.01)。结论:针刺三阴交穴、关元穴均可降低子宫收缩波个数和子宫活动度,抑制子宫平滑肌的异常收缩反应。分析其效应的部分机制是通过调节子宫靶器官组织的阿片肽受体水平,进而达到针刺镇痛的作用。Objective:To compare the different effect of needling SP6and CV4on dysmenorrhea rats of cold congealing type,by observing the uterus contraction level,and the changes ofκopioid receptor andμopioid receptor in the spinal cord and uterus tissues.Methods:64female SD rats were randomly divided into the saline control group,the model group,SP6group(needling acupoint SP6),and CV4group(needling acupoint CV4),with16rats in each group.The models were established by subcutaneously injecting Estradiol Benzoate and being exposed to freezing environment.One hour later after the injection of Estradiol Benzoate on the10th day,0.7ml/100g Urethane was given by intramuscular injection for anesthesia.BL-420F was used to record the uterus contraction.Real Time-PCR was applied to test the amount ofκopioid receptor andμopioid receptor in the spinal cord and uterus tissues.Results:In terms of uterine contraction:compared to saline control group,the amount of the uterine contraction waves,peak wave values,and uterine activities were increased significantly in the model group,group SP6,and group CV4(P<0.05or P<0.01);in which the needling groups improved significantly compared to the model group(P<0.05or P<0.01).In terms of the changes ofκopioid receptor andμopioid receptor,there was no significant difference onκandμopioid receptors in the spinal cord among the four groups;There was no significant difference on theκopioid receptor in the uterus among the groups;compared to the saline control group,μopioid receptor in the uterus increased significantly in group CV4(P<0.05);compared to the model group,μopioid receptor in the uterus increased significantly in both group SP6and group CV4(P<0.05or P<0.01).Conclusion:Needling on SP6and CV4can both reduce the amount of uterus contraction waves and contraction degree to alleviate abnormal uterus contraction.The mechanism may be achieved by regulating the endogenous opioid receptor in uterus tissues.

关 键 词：寒凝证类痛经模型 子宫收缩程度 Κ受体 Μ受体 三阴交穴 关元穴 

分 类 号：R245.2[医药卫生—针灸推拿学] R271.113[医药卫生—中医临床基础]