日本血吸虫高迁移率族蛋白B1诱导小鼠RAW264.7巨噬细胞M1型极化  

M1 TYPE POLARIZATION OF MURINE RAW264.7 MACROPHAGES INDUCED BY HIGH MOBILITY GROUP PROTEIN B1 OF SCHISTOSOMA JAPONICUM

作　　者：艾敏 史晓娜 苑纯秀[1,3] 赵小超[1] 李玉梅 侯旖旎 冯新港 AI Min;YUAN Chun-xiu;FENG Xin-gang;SHI Xiao-na;ZHAO X ia o -cW;LI Yu-mei;HOU Yi-ni(Key Laboratory of Animal Parasitology of Ministry of Agriculture, Shanghai Veterinary Research Institute, CAAS, Shanghai 200241,China;College of Life and Environment Sciences, Shanghai Normal University, Shanghai 200234, China;Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou 225009, China)

机构地区：[1]中国农业科学院上海兽医研究所农业部动物寄生虫学重点开放实验室,上海200241 [2]上海师范大学生命与环境科学学院,上海200234 [3]江苏高校动物重要疫病与人兽共患病防控协同创新中心,扬州225009

出　　处：《中国动物传染病学报》2017年第5期53-59,共7页Chinese Journal of Animal Infectious Diseases

基　　金：国家重点基础研究发展计划(2015CB150303)

摘　　要：通过分析日本血吸虫高迁移率族蛋白B1(Schistosoma japonicum high mobility group protein B1,SjHMGB1)刺激的巨噬细胞的表型相关分子的表达情况,研究SjHMGB1诱导巨噬细胞向M1型极化的功能。分别用LPS及IFN-γ和IL-4诱导小鼠RAW264.7巨噬细胞成M1型和M2型作为阳性对照组,以SjHMGB1诱导的巨噬细胞为实验组。分别利用Griess法、FCM、RTPCR和酶活性检测巨噬细胞中iNOS、CD16/32、ArginaseⅠ、CD206等主要标志分子。结果与空白对照组相比,SjHMGB1刺激组和M1型阳性对照组巨噬细胞NO分泌显著增加,精氨酸酶活性变化不显著,M2型巨噬细胞呈现相反的表型。流式结果显示SjHMGB1刺激组和M1型阳性对照组的巨噬细胞诱导型一氧化氮合成酶(iNOS)和CD16/32表达上调,而CD206变化不明显。RT-PCR结果显示SjHMGB1蛋白刺激组和M1型阳性对照组的巨噬细胞iNOS表达上调,而arginaseⅠ和CD206变化不明显;M2型巨噬细胞arginaseⅠ表达水平明显升高,CD206表达上调。结果表明SjHMGB1能够诱导巨噬细胞往M1型巨噬细胞分化。The role of Schistosoma japomcum high mobility group protein B1(5/HMGB1)in the induction of macrophages to Ml type polarization was investigated through the analysis of expression of phenotype-related molecules on stimulated macrophages.The mouse RAW264.7macrophages induced by LPS and IFN-7or IL-4respectively to Ml or M2type were set as positive control groups.Griess,FCM,RT-PCR and enzyme activities were analyzed to detect the expression of major marker molecules including iNOS,CD16/32,arginase I and CD206in macrophages.As compared with the positive control groups,a few changes were observed,including significant increase of the NO secretion of macrophages in^*HMGB1treatment group and Ml group,slight increase in the activity of arginase I and opposite phenotype of M2type macrophages.In addition,flow cytometry analysis showed significant up-regulation of the expression of iNOS and CD16/32of macrophages in5/HMGB1group and Ml group except CD206.RT-PCR results also indicated up-regulated expression of iNOS in5/HMGB1group and Ml group as well as CD206and enzyme activities of M2macrophages.However,the expression level and arginase I did not change significantly.In conclusion,S/HMGBl induced the differentiation of macrophages into Ml type polarization.

关 键 词：SjHMGB1蛋白 M1型巨噬细胞 精氨酸酶 诱导型一氧化氮合成酶 

分 类 号：S852.735[农业科学—基础兽医学]