小麦农家品种‘红蚰子’苗期白粉病抗性鉴定和遗传分析  

Identification and genetic analysis of powdery mildew resistance at seedling stage in ‘Hongyouzi' Chinese wheat landrace

作　　者：曹燕威 许红星[1] 李秀全[3] 安调过[1] CAO Yanwei;XU Hongxing;LI Xiuquan;AN Diaoguo(Center for Agricultural Resources Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences,Shijiazhuang 050022, China;Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China;University of Chinese Academy of Sciences, Beijing 100049, China)

机构地区：[1]中国科学院遗传与发育生物学研究所农业资源研究中心,石家庄050022 [2]中国科学院大学,北京100049 [3]中国农业科学院作物科学研究所,北京100081

出　　处：《中国生态农业学报》2017年第12期1842-1847,共6页Chinese Journal of Eco-Agriculture

基　　金：河北省自然科学基金项目(C2016503016)资助~~

摘　　要：白粉病是小麦的重要病害之一,优异抗病种质的鉴定和利用是控制该病害的有效措施。当前,我国小麦生产中的多数栽培品种和品系对白粉病表现为感病,可利用的优异抗性基因为数不多,因此,很有必要发掘新的有效抗源和抗性基因。我国小麦农家品种‘红蚰子’(京2350)苗期对国内38个小麦白粉病原菌菌株中的34个表现为高抗至免疫,且成株期对石家庄地区的田间混合白粉病原菌表现为免疫或近免疫。为了进一步研究‘红蚰子’抗白粉病遗传特点,发掘并利用其中的优异抗白粉病基因,本研究利用白粉菌E09菌株,分别对‘红蚰子’与感病亲本‘铭贤169’和‘辉县红’组合各自的F_1、F_2后代植株和F_(2:3)株系,进行苗期抗性鉴定和抗性遗传分析。结果表明,‘红蚰子’与‘铭贤169’和‘辉县红’组合的所有F_1均感病,各自F_2后代植株中抗病植株与感病植株的比例均符合1∶3分离比,且各自F_(2:3)株系中纯合抗病株系∶杂合株系∶纯合感病株系的比例均符合1∶2∶1的比例。因此,‘红蚰子’对E09菌株的抗性由1对隐性基因控制,暂时命名为PmHYZ。‘红蚰子’是我国农家品种中优异的白粉病抗源,研究并发掘其中的抗性基因,将为其在抗病育种中的有效利用奠定基础。Powdery mildew,caused by Blumeria graminis f.sp.tritici(Bgt),is a devastating foliar disease of wheat.The identification and utilization of elite resistant germplasm is an effective control measure of the disease.At present,most wheat cultivars/lines in China's wheat production are susceptible to powdery mildew.Moreover,few powdery mildew resistance genes are used in China.Therefore,it is essential to identify more effective and elite resistance sources and genes.The'Hongyouzi'Chinese wheat landrace('Jing2350')has shown immune to high resistant of34out of38Bgt isolates at seedling stage and immune or nearly immune in the field to a composites of Bgt isolates at mature stage in Shijiazhuang,China.To further study the resistance feature and identify the resistance gene(s)of'Hongyouzi',genetic analysis of F1plants,F2populations and their derived F2∶3families respectively from crosses of'Huixianhong'×'Hongyouzi'and'Hongyouzi'×'Mingxian169'were conducted at seedling stage.The results showed that all F1plants derived from the combinations of'Huixianhong'×'Hongyouzi'and'Hongyouzi'×'Mingxian169'were susceptible to Bgt isolate E09.The ratios of resistant F2plants to susceptible plants conformed as1︰3and those of homozygous resistant F2∶3lines︰heterozygous lines︰homozygous susceptible lines conformed as1︰2︰1.These results indicated that the resistance of'Hongyouzi'to Bgt isolate E09was conferred by a single recessive resistance gene,temporarily named PmHYZ.'Hongyouzi'was the elite powdery mildew resistance sources of Chinese wheat landraces,thus it was useful to effectively use elite resistance source and resistance gene in breeding resistant crops.

关 键 词：小麦 白粉病 农家品种 红蚰子 遗传分析 

分 类 号：S332.2[农业科学—作物遗传育种]