黄淮海地区大豆主栽品种对8个大豆疫霉菌株的抗性评价  

Resistance Evaluation to Eight Phytophthora sojae Isolates for Major Soybean Cultivars in Huang-Huai-Hai Rivers Valley

作　　者：李晓那 孙石[2] 钟超[2] 韩天富[1,2] LI Xiao-Na;SUN Shi;ZHONG Chao;HAN Tian-Fu(College of Agriculture, Northeast Agricultural University, Harbin 150030, China;Key Laboratory of Soybean Biology (Beijing) / Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China)

机构地区：[1]东北农业大学农学院,黑龙江哈尔滨150030 [2]中国农业科学院作物科学研究所/农业部北京大豆生物学重点实验室,北京100081

出　　处：《作物学报》2017年第12期1774-1783,共10页Acta Agronomica Sinica

基　　金：国家自然科学基金项目(31471521);国家现代农业产业技术体系建设专项(CARS-04);国家公益性行业(农业)科研专项经费项目(201303018);中国农业科学院农业科技创新工程资助~~

摘　　要：随着麦茬免耕栽培技术的推广应用,黄淮海地区麦后夏播大豆生产中疫霉根腐病呈加重趋势。了解该地区大豆主栽品种对疫霉根腐病的抗性和筛选抗病亲本,对培育新的高产广适抗病品种具有重要意义。本研究利用8个具有不同毒力的大豆疫霉菌株,采用下胚轴创伤接种法,对20世纪50年代以来黄淮海地区审定、推广的140个大豆主栽品种进行接种鉴定。表明除6个品种对8个菌株均无抗性外,其余134个品种分别抗1~8个大豆疫霉菌株,占鉴定品种总数的95.7%,其中抗6~8个以上菌株的品种有83个,占鉴定品种总数的59.3%。以14个鉴别寄主的抗病反应型为参照,发现134个品种对8个大豆疫霉菌株共产生65种反应型,其中19个品种产生的5种反应型与已知单基因或2个单基因组合反应型相同;115个品种产生的60种反应型与含有已知单基因或2个单基因组合的反应型不同,推测可能含有新的抗病基因或基因组合。根据研究结果合理选择亲本,可培育出聚合多个抗性基因且综合性状优良的大豆新品种。With the application of wheat stubble no-tillage cultivation technology,the occurrence rate of phytophthora root rot of soybean is getting higher in the Huang-Huai-Hai Rivers Valley.Therefore,it is crucial to investigate the resistance of the widely-grown soybean cultivars to phytophthora root rot and to screen disease-resistant resources for soybean improvement.In the current study,a total of140soybean cultivars,widely-grown in Huang-Huai-Hai Rivers Valley since the1950s,were investigated for their response to eight strains of Phytophthora sojae through the hypocotyl inoculation method.Except that six cultivars were susceptible to all strains,134cultivars(95.7%)were resistant to1-8strains,including83cultivars(59.3%)resistant to6-8strains.A total of65reaction types in response to the eight strains were identified from the134resistant cultivars by means of14reference genotypes.Five reaction types produced by19cultivars fit the reaction-type profile of a known resistance gene or the combination of two genes.The remaining60reaction types by115cultivars were different from all reaction types of a single resistance gene or the combinations of two genes,indicating that theses cultivars might carry novel resistance gene(s)/gene combination(s)against P.sojae.These results provide a guide in parent selection for pyramiding resistance genes in soybean cultivars with good agronomic backgrounds.

关 键 词：大豆 疫霉根腐病 大豆疫霉 抗性基因 基因推导 

分 类 号：S435.651[农业科学—农业昆虫与害虫防治]