不同来源的间充质干细胞线粒体结构及活性鉴定  

Mitochondrial structure and activities of mesenchymal stem cells derived from different human tissues

作　　者：周金萍 祝献民 曾桥[3] 薛志刚[1,3] ZHOU Jin-ping;ZHU Xian-min;ZENG Qiao;XUE Zhi-gang(Dept, of Regenerative Medicine, School of Medicine, Tongji University, Shanghai 200092, China;School of Life Sciences and Technology, Tongji University, Shanghai 200092, China;Translational Center for Stem Cell Research, Tongji Hospital, Tongji University, Shanghai 200065, China)

机构地区：[1]同济大学医学院再生医学系,上海200092 [2]同济大学生命科学与技术学院,上海200092 [3]同济大学附属同济医院干细胞临床转化中心,上海200065

出　　处：《同济大学学报（医学版）》2017年第5期13-17,共5页Journal of Tongji University(Medical Science)

基　　金：国家自然科学基金重点项目(81430026);上海市科委基础研究项目(16JC1404700)

摘　　要：目的培养脂肪、骨髓、牙髓及脐带4种不同来源的间充质干细胞,并鉴定其线粒体结构和活性。方法通过流式细胞术、透射电子显微镜及细胞代谢分析仪等技术分别检测各种间充质干细胞的纯度、内部线粒体的超微结构及呼吸耗氧量。结果脂肪、骨髓、牙髓及脐带4种间充质细胞均成纤维状贴壁生长,同时表达CD73、CD90及CD105,不表达CD34、CD11b、CD19、CD45及HLA-DR;ASC与BMSC较DPSC与UCDSC线粒体结构成熟,代谢水平高。结论脂肪、骨髓、牙髓及脐带来源的细胞均具有间充质干细胞的特点,但其线粒体结构和活性等方面存在差异。ObjectiveTo compare the mitochondrial structure and activities of mesenchymal stem cells(MSCs)derived from different human tissues.MethodsMesenchymal stem cells were isolated from human adipose tissue,bone marrow,dental pulp and umbilical cord.The isolated MSCs were cultured and proliferated in vitro,and the purity was assayed by Flow Cytometry.The ultrastructure and metabolic activity of MSCs were detected by transmission electron microscopy and XFeExtracellular Flux Analyzers,respectively.ResultsMSCs derived from4tissues presented in culture as adherent growth and a spindle cell morphology,the cells positively expressed CD73,CD90and CD105,and negatively expressed CD34,CD11b,CD19,CD45and HLA DR.Compared with MSCs from dental pulp and umbilical cord,the mitochondria of MSCs from adipose tissue and bone marrow had more mature morphology and higher metabolic activity.ConclusionPluripotent stem cells derived from different tissues show similar characteristics,however,may have different ultrastructure and metabolic activity of mitochondria in MSCs of different origin.

关 键 词：间充质干细胞 细胞培养 结构 活性 

分 类 号：Q244[生物学—细胞生物学]