心肌缺血-再灌注所致单相动作电位振幅改变与心肌损伤程度的相关性研究  

Correlation between myocardial ischemia-reperfusion-induced monophasic action potential amplitude change and myocardial damage

作　　者：刘盈盈[1] 李勤[2] LIU Ying ying;LI Qin(Electrocardiogram Room, the First People's Hospital of Yibin City in Sichuan Province, Yibin City, Sichuan Province, 644000, China;Department of Cardiovascular Medicine, the First People's Hospital of Yibin City in Sichuan Province, Yibin City, Sichuan Province, 644000, China)

机构地区：[1]四川省宜宾市第一人民医院心电图室,四川宜宾644000 [2]四川省宜宾市第一人民医院心血管内科,四川宜宾644000

出　　处：《海南医学院学报》2017年第17期2320-2322,2327,共4页Journal of Hainan Medical University

基　　金：四川省医学会课题项目(Q14049)~~

摘　　要：目的:研究心肌缺血-再灌注所致单相动作电位振幅(MAPA)改变与心肌损伤程度的相关性。方法:选择新西兰家兔作为实验动物并随机分为对照组和缺血再灌注组(I/R组),建立心肌缺血再灌注损伤模型后分离心脏并在Langendorff灌流系统内测定心肌内膜层、中膜层、外膜层的MAPA;另取血清标本及心肌组织,测定心肌损伤分子的含量。结果:I/R组动物心肌内膜层、中膜层、外膜层的MAPA水平均显著高低于对照组;I/R组动物血清中CK-MB、cTnI、cTnT、MDA的含量以及心肌组织中Bax、Caspase-3、Caspase-9的mRNA表达量均显著高于对照组且与心肌内膜层、中膜层、外膜层的MAPA水平呈负相关,血清中SOD、GSH-Px、HO-1的含量以及心肌组织中Bcl-2、Bcl-xL的mRNA表达量显著低于对照组且与心肌内膜层、中膜层、外膜层的MAPA水平呈正相关。结论:心肌缺血-再灌注会引起MAPA降低且与心肌氧化应激损伤及凋亡密切相关。Objective:To study the correlation between myocardial ischemia reperfusion induced monophasic action potential amplitude(MAPA)change and myocardial damage.Methods:New Zealand rabbits were selected as experimental animals and randomly divided into control group and ischemia reperfusion group(I/R group),myocardial ischemia reperfusion injury models were established,then the heart was separated and the MAPA of myocardial intima layer,media layer and outer layer were determined in Langendorff perfusion system;serum samples and myocardial tissue were collected to determine the contents of myocardial injury molecules.Results:MAPA levels of myocardial intima layer,media layer and outer layer of I/R group were significantly lower than those of control group;CK MB,cTnI,cTnT and MDA contents in serum as well as Bax,Caspase3and Caspase9mRNA expression in myocardial tissue of I/R group were significantly higher than those of control group and negatively correlated with MAPA levels of myocardial intima layer,media layer and outer layer while SOD,GSH Px and HO1contents in serum as well as Bcl2and Bcl xL mRNA expression in myocardial tissue were significantly lower than those of control group and positively correlated with MAPA levels of myocardial intima layer,media layer and outer layer.Conclusion:Myocardial ischemia reperfusion can induce the decrease of MAPA and is closely related to myocardial oxidative stress injury and apoptosis.

关 键 词：心肌缺血再灌注 单相动作电位 氧化应激 细胞凋亡 

分 类 号：R542.2[医药卫生—心血管疾病]