利用原子力显微镜峰值力定量测量模式分析人间充质干细胞早期分化的力学表型  

Mechanical phenotype during early differentiation of human mesenchymal stem cells by quantitative measurement of peak force in atomic force microscopy

作　　者：刘知晓 张金金[1] 王鑫艳 陈峰[4] 胡钧[1,2] 吕军鸿 LIU Zhi-xiao;ZHANG Jin-jin;WANG Xin-yan;CHEN Feng;HU Jun;Jun hong(Laboratory of Physical Biology, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, China;Department of Inorganic Chemistry, School of Chemistry and Chemical Engineering, University of Chinese Academy of Sciences, Beijing 100049, China;National Center for Protein Science Shanghai, Shanghai 200031, China;Beijing Shijitan Hospital, Capital Medical University, Beijing 100038, China)

机构地区：[1]中国科学院上海应用物理研究所物理生物学实验室,上海201800 [2]中国科学院大学化学与化工学院无机化学教研室,北京100049 [3]国家蛋白质科学中心(上海),上海200031 [4]首都医科大学附属北京世纪坛医院,北京100038

出　　处：《第二军医大学学报》2017年第10期1261-1266,共6页Academic Journal of Second Military Medical University

基　　金：国家自然科学基金(11474298;11405250);中国科学院前沿科学重点研究方向基金(QYZDJ-SSW-SLH019)~~

摘　　要：目的建立通过力学表型来灵敏反映人间充质干细胞分化早期微小力学性质变化的技术。方法使用基于原子力显微镜峰值力定量纳米力学作图(peak force quantitative nanomechanical mapping,PF-QNM)技术,测量在不同浓度氯化锂诱导下人间充质干细胞的力学变化过程。结果 4mmol/L和30mmol/L氯化锂处理干细胞48h后其纳米力谱就有显著的差异;但要到72h之后,平均杨氏模量才能区分不同浓度氯化锂处理所导致的差别。结论纳米力谱比平均杨氏模量更能反映干细胞分化早期的力学性质变化。基于纳米力谱的力学表型可以作为物理生物学标记来鉴定干细胞的早期分化。ObjectiveTo establish a technique for sensitively reflecting the micro mechanical changes of human mesenchymal stem cells(hMSCs)in their early differentiation process by mechanical phenotype.MethodsBased on atomic force microscopy(AFM),peak force quantitative nanomechanical mapping(PF QNM)technique was used to measure the nanomechanical spectroscopy of hMSCs during differentiation induced by different concentrations of lithium chloride.ResultsThe nanomechanical spectroscopy of hMSCs induced by4mmol/L and30mmol/L lithium chloride was significantly different after48h,while there was significant difference in Young’s modulus after72h.ConclusionNanomechanical spectroscopy is superior to Young’s modulus in reflecting the change of mechanical properties of hMSCs in their early differentiation phase.Mechanical phenotype based on nanomechanical spectroscopy can be used as a physical biomarker to identify the hMSCs at their early differentiation stage.

关 键 词：间质干细胞 力学表型 纳米力谱 细胞分化 

分 类 号：R329.29[医药卫生—人体解剖和组织胚胎学]